芪红通络颗粒对大鼠脑缺血再灌注损伤保护作用及其机制研究
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安徽省高等学校自然科学研究项目(KJ2019A0446);安徽省自然科学基金(1608085MH161)


Protective Effect of Qihong Tongluo Granule Against Cerebral Ischemia/Reperfusion Injury in Rats and Its Mechanism
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    摘要:

    目的 观察芪红通络颗粒对大鼠脑缺血再灌注损伤的保护作用,并初步探讨其作用机制。方法 将SD大鼠随机分为假手术组,模型组,尼莫地平组,芪红通络颗粒高、中、低剂量(10.8、5.4、2.7 g/kg)组,采用线栓法建立大鼠局灶性脑缺血再灌注损伤模型。各组大鼠术前连续3 d、术后连续2 d灌胃给药,每日1次。术后72 h采用Longa法进行神经功能缺损评分,TTC染色法测定脑梗死体积,HE染色观察脑组织病理学改变,比色法测定脑组织丙二醛(malondialdehyde, MDA)含量和超氧化物歧化酶(superoxide dismutase, SOD)、谷胱甘肽过氧化物酶(glutathion peroxidase,GSH- Px)活力,Western blot法测定脑组织核因子E2相关因子2(nuclear factor erythroid- 2 related factor 2, Nrf2)和血红素氧合酶- 1(heme oxygenase 1, HO- 1)蛋白表达水平。结果 与模型组比较,芪红通络颗粒高、中、低剂量组脑梗死体积显著缩小(P<0.05);高、中剂量组神经行为学障碍症状明显减轻(P<0.05),脑组织病理改变明显减轻;高、中、低剂量组脑组织中MDA含量明显降低,SOD和GSH- Px活力明显增加(P<0.05);高、中剂量组脑组织中Nrf2和HO- 1蛋白表达水平明显升高(P<0.05)。结论 芪红通络颗粒对大鼠脑缺血再灌注损伤具有较好的保护作用,其机制可能与其激活Nrf2/HO- 1信号通路,抑制氧化应激反应,从而产生抗氧化作用有关。

    Abstract:

    Objective To investigate the protective effect of Qihong Tongluo Granule against cerebral ischemia/reperfusion injury in rats and its preliminary mechanism of action. Methods Sprague- Dawley rats were randomly divided into sham- operation group, model group, nimodipine group, and high- , middle- , and low- dose (10.8,5.4, and 2.7 g/kg) Qihong Tongluo Granule groups. The suture method was used to establish a rat model of focal cerebral ischemia/reperfusion injury. The rats were administered once a day by gavage for 3 consecutive days before surgery and 2 consecutive days after surgery. At 72 hours after surgery, the Longa method was used to determine neurological deficit score; TTC staining was used to measure cerebral infarct volume; HE staining was used to observe the pathological changes of brain tissue; colorimetry was used to measure the content of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH- Px) in brain tissue; Western blot was used to measure the protein expression levels of nuclear factor erythroid 2- related factor 2 (Nrf2) and heme oxygenase- 1 (HO- 1) in brain tissue. Results Compared with the model group, the high- , middle- , and low- dose Qihong Tongluo Granule groups had a significant reduction in cerebral infarct volume (P<0.05); the high- and middle- dose Qihong Tongluo Granule groups had significantly alleviated symptoms of neurobehavioral disturbance (P<0.05) and pathological changes of brain tissue; the high- , middle- , and low- dose Qihong Tongluo Granule groups had a significant reduction in the content of MDA and significant increases in the activities of SOD and GSH- Px in brain tissue (P<0.05); the high- and middle- dose Qihong Tongluo Granule groups had significant increases in the protein expression levels of Nrf2 and HO- 1 in brain tissue (P<0.05). Conclusion Qihong Tongluo Granule exerts a good protective effect against cerebral ischemia/reperfusion injury in rats, possibly by activating the Nrf2/HO- 1 signaling pathway, inhibiting oxidative stress response, and thus producing an antioxidant effect.

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高玉菊,杨满琴,王 丽,罗胜勇.芪红通络颗粒对大鼠脑缺血再灌注损伤保护作用及其机制研究[J].安徽中医药大学学报,2021,40(3):64-69

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  • 在线发布日期: 2021-06-16