六味补气胶囊对慢性阻塞性肺疾病模型大鼠肺功能及炎症因子的影响
Effect of Liuwei Buqi Capsule on Pulmonary Function and Inflammatory Factors in a Rat Model of Chronic Obstructive Pulmonary Disease
  
DOI:
中文关键词:  六味补气胶囊  慢性阻塞性肺疾病  肺功能  炎症因子
英文关键词:Liuwei Buqi Capsule  Chronic obstructive pulmonary disease  Pulmonary function  Inflammatory factor
基金项目:安徽省自然科学基金项目(1708085MH222)
作者单位
方 芳,刘向国,李 敏,徐晴雯,华 华,于芳春,任冯春 安徽中医药大学安徽 合肥 230012 
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中文摘要:
      目的 探讨六味补气胶囊对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)模型大鼠肺功能及炎症因子的影响。方法 将SD大鼠按随机数字表法分为正常组,模型组,桂龙咳喘宁组和六味补气胶囊高、中、低剂量组,每组10只。观察记录大鼠一般状况;采用肺功能检测仪检测用力肺活量(forced vital capacity,FVC)、第3秒用力呼气容积(forced expiratory volume in 0.3 seconds,FEV0.3)、FEV0.3占FVC的比值(FEV0.3/FVC)和呼气峰流速(peak expiratory flow,PEF);采用苏木精-伊红染色观察支气管肺组织的病理变化;采用ELISA法检测血清及肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中白介素-8(interleukin 8,IL-8)、白介素-1β(interleukin 1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的水平。结果 与正常组比较,模型组大鼠一般状况较差,肺功能降低,支气管黏膜皱襞减少、甚至脱落,黏膜下炎症细胞浸润增加,血清及BALF中IL-8、IL-1β、TNF-α水平均明显升高(P<0.05);与模型组比较,六味补气胶囊各剂量组和桂龙咳喘宁组大鼠一般状况及肺功能均有不同程度改善,肺组织病理改变较轻,血清及BALF中IL-8、IL-1β、TNF-α的水平均明显下降(P<0.05);与桂龙咳喘宁组比较,六味补气胶囊低剂量组大鼠一般状况较差,肺功能降低,肺组织病理改变增加,血清及BALF中IL-8、IL-1β、TNF-α水平均明显升高(P<0.05);六味补气胶囊中剂量组各项指标差异无统计学意义(P>0.05);六味补气胶囊高剂量组大鼠一般状况和肺功能改善较明显,肺组织病理改变较少,血清及BALF中IL-8、IL-1β、TNF-α的水平均明显下降(P<0.05);与六味补气胶囊低剂量组比较,六味补气胶囊中、高剂量组一般状况和肺功能均有不同程度改善,肺组织病理改变较少,血清及BALF中IL-8、IL-1β、TNF-α的水平均明显下降(P<0.05);与六味补气胶囊中剂量比较,六味补气胶囊高剂量组一般状况和肺功能改善较明显,肺组织病理改变较少,血清及BALF中IL-8、IL-1β、TNF-α的水平均明显下降(P<0.05)。结论 六味补气胶囊可以减轻COPD模型大鼠肺组织病理学变化,改善气道炎症,增加肺通气功能。
英文摘要:
      Objective To investigate the effect of Liuwei Buqi Capsule on pulmonary function and inflammatory factors in a rat model of chronic obstructive pulmonary disease (COPD). Methods Sprague-Dawley rats were divided into normal group, model group, Guilong Kechuanning group, and high-, middle-, and low-dose Liuwei Buqi Capsule groups using a random number table, with 10 rats in each group. General status of the rats were recorded; a pulmonary function apparatus was used to measure forced vital capacity (FVC), forced expiratory volume in 0.3 seconds (FEV0.3), FEV0.3/FVC ratio, and peak expiratory flow; HE staining was used to observe the pathological changes of bronchopulmonary tissue; ELISA was used to measure the levels of interleukin 8 (IL-8), interleukin 1β (IL-1β), and tumor necrosis factor-α (TNF-α) in serum and bronchoalveolar lavage fluid (BALF). Results Compared with the normal group, the model group had poor general status, reduced pulmonary function, reduction or even shedding of the bronchial mucosa, an increase in submucosal inflammatory cell infiltration, and significant increases in the levels of IL-8, IL-1β, and TNF-α in serum and BALF (P<0.05). Compared with the model group, the high-, middle-, and low-dose Liuwei Buqi Capsule groups and the Guilong Kechuanning group had varying degrees of improvement in pulmonary function, slight pathological changes of lung tissue, and significant reductions in the levels of IL-8, IL-1β, and TNF-α in serum and BALF (P<0.05). Compared with the Guilong Kechuanning group, the low-dose Liuwei Buqi Capsule group had poor general status, reduced pulmonary function, increased pathological changes of lung tissue, and significant increases in the levels of IL-8, IL-1β, and TNF-α in serum and BALF (P<0.05). There were no significant differences in these indices between the high-, middle-, and low-dose Liuwei Buqi Capsule groups (P>0.05). The high-dose Liuwei Buqi Capsule group had significant improvement in general status and pulmonary function, slight pathological changes of lung tissue, and significant reductions in the levels of IL-8, IL-1β, and TNF-α in serum and BALF (P<0.05). Compared with the low-dose Liuwei Buqi Capsule group, the high- and middle-dose Liuwei Buqi Capsule groups had varying degrees of improvement in general status and pulmonary function, slight pathological changes of lung tissue, and significant reductions in the levels of IL-8, IL-1β, and TNF-α in serum and BALF (P<0.05). Compared with the middle-dose Liuwei Buqi Capsule group, the high-dose Liuwei Buqi Capsule group had significant improvements in general status and pulmonary function, slight pathological changes of lung tissue, and significant reductions in the levels of IL-8, IL-1β, and TNF-α in serum and BALF (P<0.05). Conclusion In a rat model of COPD, Liuwei Buqi Capsule can alleviate the pathological changes of lung tissue, improve airway inflammation, and increase pulmonary ventilation function.
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