木鳖子炮制前后脂肪油提取工艺及气相色谱-质谱联用分析研究
Fatty Oil Extraction Process and Analysis of Gas Chromatography-Mass Spectroscopy Before and After Processing of Momordicae Semen
  
DOI:
中文关键词:  木鳖子  脂肪油  炮制  气相色谱-质谱联用
英文关键词:Momordicae Semen  Fatty oil  Processing  Gas chromatography-mass spectroscopy
基金项目:国家重点研发计划项目(2018YFC1707004);浙江中医药大学省重点建设高校优势特色学科(中药学)开放基金项目(ZYAOXZD2019005);安徽省高校优秀青年骨干人才出国访学研修项目(gxgwfx2018043)
作者单位
江 瑜,姚梦雪,张姗姗,苏 敏,范兰兰,汪小莉,洪 燕,桂双英,韩燕全 1. 安徽中医药大学第一附属医院 国家中医药管理局中药制剂三级实验室 中药复方安徽省重点实验室 现代药物制剂安徽省工程技术中心安徽 合肥 2300312.安徽中医药大学安徽 合肥 230012 
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中文摘要:
      目的 优选木鳖子脂肪油的提取工艺,明确其炮制前后脂肪油成分“质”“量”差异,为木鳖子的炮制机制和质量控制提供依据。方法 采用L9(34)正交设计,以索氏提取法提取木鳖子脂肪油,通过设计料液比、提取时间、提取温度的不同因素水平,考察木鳖子脂肪油提取工艺;采用气相色谱-质谱联用法对木鳖子和木鳖子霜的脂肪油成分进行分析与鉴定,并通过多元统计方法分析木鳖子制霜前后脂肪油成分的变化,确定其差异性脂肪油。结果 优选出木鳖子脂肪油的最佳提取工艺为料液比为1∶40,提取时间为6 h,提取温度为85 ℃。木鳖子制霜后脂肪油的总量下降,但其成分类别无明显变化,共鉴定出14个脂肪油成分。通过对炮制前后14个脂肪油成分进行主成分分析和正交偏最小二乘判别分析,结合变量重要性投影贡献值,筛选得到8个含量具有显著变化的脂肪油成分。结论 木鳖子制霜后去油率为13%左右,脂肪油种类无明显变化,但多个脂肪油成分相对含量具有显著变化,木鳖子制霜后脂肪油成分变化对其质量和药效的影响需要进一步研究。
英文摘要:
      Objective To optimize the extraction process for fatty oil in Momordicae Semen, to investigate the changes in the "quality" and "quantity" of fatty oil constituents after processing, and to provide a basis for the processing mechanism and quality control of Momordicae Semen. Methods The L9 (34) orthogonal design was used, and the Soxhlet extraction method was used to extract fatty oil from Momordicae Semen. The extraction process for fatty oil from Momordicae Semen was investigated based on the different factors of material-liquid ratio, extraction time, and extraction temperature. Gas chromatography-mass spectroscopy was used to analyze and identify the fatty oil constituents of Momordicae Semen and Momordicae Semen cream, and the multivariate statistical method was used to analyze the change in fatty oil constituents after the preparation of Momordicae Semen cream and identify the differential fatty oil constituents. Results The optimal extraction process was a material-liquid ratio of 1∶40, an extraction time of 6 hours, and an extraction temperature of 85 ℃. There was a reduction in the total amount of fatty oil after the preparation of Momordicae Semen cream, while there were no significant changes in each constituent. A total of 14 fatty oil constituents were identified. A principal component analysis and an orthogonal partial least squares discrimination analysis were performed for the 14 fatty oil constituents before and after processing, and with reference to the variable importance in projection value, 8 fatty oil constituents with significant changes in content were screened out. Conclusion The deoiling rate is about 13% after the preparation of Momordicae Semen cream, and there is no significant change in the type of fatty oil constituents, but there are significant changes in the relative content of several fat oil constituents. Further studies are needed to investigate the influence of the change in fatty oil constituents on the quality and efficacy of Momordicae Semen after the preparation of Momordicae Semen cream.
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