五味子乙素对脂多糖诱导的脓毒症急性肺损伤大鼠肺组织病理损伤、炎症反应和核因子-κB表达的影响
Effect of Schisandrin B on Pathological Injury, Inflammatory Response, and Nuclear Factor-Kappa B Expression in Lung Tissue of Rats with Sepsis and Acute Lung Injury Induced by Lipopolysaccharide
  
DOI:
中文关键词:  五味子乙素  脂多糖  脓毒症急性肺损伤  核因子-κB
英文关键词:Schisandrin B  Lipopolysaccharide  Sepsis with acute lung injury  Nuclear factor-kappa B
基金项目:河南省科技攻关计划项目(172102310585)
作者单位
钟春蕾,黄国涛,张志强 1.新乡医学院第一附属医院呼吸科河南 新乡 4530002.新乡医学院第一附属医院心内科河南 新乡 453000 
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中文摘要:
      目的 观察五味子乙素对脂多糖(lipopolysaccharide,LPS)诱导的脓毒症急性肺损伤大鼠肺组织病理损伤、炎症反应和核因子 -κB (nuclear factor kappa-B,NF-κB)表达的影响。方法 采用气管滴注40 mg/kg LPS制备大鼠急性肺损伤大鼠模型,手术前1 h腹腔注射80 mg/kg 五味子乙素进行干预治疗,测定大鼠肺湿/干质量比,BCA试剂盒测定大鼠支气管肺泡灌洗液(bronchoalceolar lavage fluid,BALF)总蛋白,牛鲍氏计数板计算 BALF中白细胞数,苏木精-伊红染色观察大鼠肺组织损伤情况,ELISA法检测大鼠肺组织中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-6(interleukin-6,IL-6)含量,相关试剂盒检测大鼠肺组织中丙二醛(malondialdehyde,MDA)和超氧化物歧化酶(superoxide dismutase,SOD)含量,蛋白质印迹法检测大鼠肺组织中NF-κB p65、磷酸核因子- κB 抑制蛋白(phosphorylation inhibitor of nuclear factor kappa-B,pIκBα)和Toll样受体4(Toll-like receptor 4,TLR4)的表达水平。结果 与模型组相比,五味子乙素组大鼠肺湿/干质量比、BALF中总蛋白含量及白细胞数均显著减少(P<0.05),病理损伤显著减轻,TNF-α、IL-1β、IL-6含量均显著减少(P<0.05),MDA含量显著下降,SOD活性显著升高(P<0.05),NF-κB p65、pIκBα和TLR4表达水平均显著下调(P<0.05)。结论 五味子乙素能够减轻LPS诱导的脓毒症急性肺损伤大鼠肺组织的病理损伤,抑制炎症反应和氧化应激反应,抑制NF-κB通路激活。
英文摘要:
      Objective To investigate the regulatory effect of schisandrin(Sch) B on pathological injury, inflammatory response, and nuclear factor-kappa B (NF-κB) expression in lung tissue of rats with sepsis and acute lung injury induced by lipopolysaccharide (LPS). Methods A rat model of acute lung injury was established by intratracheal instillation of 40 mg/kg LPS, and intervention treatment was performed by intraperitoneal injection of 80 mg/kg Sch B at 1 hour before surgery. The lung wet/dry mass ratio was measured; BCA kit was used to measure total protein in bronchoalveolar lavage fluid (BALF), and the abalone counter was used to calculate the number of white blood cells; HE staining was used to observe lung injury. ELISA was used to measure the content of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in lung tissue; related kits were used to measure the content of malondialdehyde (MDA) and superoxide dismutase (SOD) in lung tissue; Western blot was used to measure the expression levels of nuclear factor-kappa B p65 (NF-κB p65), pIκBα, and Toll-like receptor 4 (TLR4) in lung tissue. Results Compared with the model group, the schisandrin B group had significant reductions in lung wet/dry mass ratio, total protein and number of white blood cells in BALF, pathological injury, and the content of TNF-α, IL-1β, and IL-6 (P<0.05), as well as a significant reduction in MDA content, a significant increase in SOD content, and significant reductions in the expression of NF-κB p65, pIκBα, and TLR4 (P<0.05). Conclusion In rats with sepsis and acute lung injury induced by LPS, schisandrin B can alleviate pathological injury of lung tissue and inhibit inflammatory response, oxidative stress response, and activation of the NF-κB pathway.
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