基于网络药理学研究丹蛭降糖胶囊治疗糖尿病的分子机制
Molecular Mechanism of Danzhi Jiangtang Capsule in Treatment of Diabetes: An Analysis Based on Network Pharmacology
  
DOI:
中文关键词:  丹蛭降糖胶囊  糖尿病  网络药理学  作用机制
英文关键词:Danzhi Jiangtang Capsule  Diabetes  Network pharmacology  Mechanism of action
基金项目:安徽省自然科学基金项目(1908085MH269)
作者单位
庞静雯,王丹慧,徐梅月,张艳春,张祖志 1. 安徽中医药大学药学院安徽 合肥 2300122. 中药复方安徽省重点实验室安徽 合肥 230012 
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中文摘要:
      目的 采用网络药理学方法研究丹蛭降糖胶囊治疗糖尿病的潜在分子机制。方法 借助中药系统药理学平台和Swiss Target Prediction数据库检索丹蛭降糖胶囊的化学成分、对应作用靶标及靶标基因。通过GeneCards获取糖尿病相关靶点,将复方活性成分靶点和糖尿病靶点构建韦恩图,以寻找交集靶点,获得丹蛭降糖胶囊治疗糖尿病的预测靶标。利用Cytoscape 3.7.2软件构建药材-化合物-靶点(基因)网络,筛选关键化合物。利用STRING网站建立交集靶点蛋白相互作用网络,选出关键靶点基因。借助R软件对共同靶点进行GO分析和KEGG通路富集分析。结果 丹蛭降糖胶囊作用于糖尿病的活性成分有25个,相关靶基因76个。GO和KEGG分析结果显示,丹蛭降糖胶囊治疗糖尿病的机制共涉及1 702条生物学过程和149条信号通路。结论 丹蛭降糖胶囊治疗糖尿病的关键成分槲皮素、木犀草素、山奈酚等可能通过对AGE-RAGE、IL-17、TNF、HIF-1等信号通路的调控,最终达到治疗糖尿病的效果。其作用机制可能与抗炎,降低氧化应激水平,参与细胞的凋亡与损伤有关。
英文摘要:
      Objective To investigate the potential molecular mechanism of Danzhi Jiangtang Capsule in the treatment of diabetes based on network pharmacology. Methods Traditional Chinese Medicine Systems Pharmacology and Swiss Target Prediction database were used to search for the chemical components, corresponding targets, and target genes of Danzhi Jiangtang Capsule. GeneCards was used to obtain the targets of diabetes, and a Venn diagram was plotted for the targets of active components and the targets of diabetes to search for intersecting targets and obtain the predictive targets of Danzhi Jiangtang Capsule in the treatment of diabetes. Cytoscape 3.7.2 software was used to construct a medicinal material-compound-target (gene) network and screen out key compounds, and STRING website was used to establish a protein-protein (PPI) network of intersecting targets and screen out key target genes. R software was used to perform GO analysis and KEGG pathway enrichment analysis of common targets. Results In Danzhi Jiangtang Capsule, there were 25 active components acting on diabetes, with 76 related target genes. GO and KEGG analyses showed that 1702 biological processes and 149 signaling pathways were involved in the mechanism of action of Danzhi Jiangtang Capsule in the treatment of diabetes. Conclusion The key components of Danzhi Jiangtang Capsule, such as quercetin, luteolin, and kaempferol, can exert a therapeutic effect on diabetes by regulating the AGE-RAGE, IL-17, TNF, HIF-1, and other signaling pathways, and its mechanism of action may be associated with anti-inflammation, reduction of oxidative stress level, and participation in cell apoptosis and injury.
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