不同穴位艾灸对腹泻模型小鼠腹泻指数及小肠AQP8、VIP及SP表达的影响
DOI:
作者:
作者单位:

作者简介:

通讯作者:

基金项目:

安徽省中医药领军人才建设项目(ZYYLJRC201911);国家自然科学基金项目(81904294);安徽高校科研创新平台团队建设项目(2015TD033)


Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
    摘要:

    目的 探讨不同穴位及配伍对腹泻模型小鼠腹泻指数的影响及艾灸干预腹泻的作用机制。方法 将昆明小鼠90只,随机分为正常组、模型组、天枢组、大肠俞组及天枢大肠俞组。采用番泻叶灌胃法建立小鼠腹泻模型,对天枢组、大肠俞组、天枢大肠俞组大鼠进行艾灸,其中天枢组选取双侧天枢穴,大肠俞组选取双侧大肠俞穴,天枢大肠俞组选取一侧天枢、大肠俞 (隔日交换对侧),观察各组小鼠72 h后腹泻指数及小肠水通道蛋白8(aquaporin protein-8,AQP8)、血管活性肠肽(vasoactive intestinal peptide,VIP)及P物质(substance P,SP)免疫阳性细胞表达的变化。结果 与正常组比较,模型组腹泻指数显著增高(P<0.05);与模型组比较,天枢组、大肠俞组、天枢大肠俞组腹泻指数显著降低(P<0.05);与天枢组、大肠俞组比较,天枢大肠俞组腹泻指数显著降低(P<0.05)。与正常组比较,模型组小肠组织AQP8免疫阳性细胞表达显著减少(P<0.05),VIP、SP免疫阳性细胞表达显著增加(P<0.05);与模型组比较,天枢大肠俞组小肠组织AQP8免疫阳性细胞表达显著增加(P<0.05),VIP、SP免疫阳性细胞表达显著减少(P<0.05)。结论 天枢与大肠俞配伍治疗腹泻具有协同效应,艾灸治疗腹泻机制可能与上调AQP8表达或抑制VIP、SP表达有关。

    Abstract:

    Objective To investigate the effect of moxibustion at different acupoints on diarrhea index in a mouse model of diarrhea and the mechanism of action of moxibustion in the treatment of diarrhea. Methods A total of 90 Kunming mice were randomly divided into normal group, model group, Tianshu group, Dachangshu group, and Tianshu+Dachangshu group. Senna was given by gavage to establish a mouse model of diarrhea. The mice in the Tianshu group were given moxibustion at Tianshu points at both sides, those in the Dachangshu group were given moxibustion at Dachangshu points at both sides, and those in the Tianshu+Dachangshu group were given moxibustion at Tianshu and Dachangshu points at one side and the other side alternatively. The groups were compared in terms of the changes in diarrhea index and the numbers of aquaporin protein-8 polypeptide (AQP8)-, vasoactive intestinal peptide (VIP)-, and substance P (SP)-positive cells in the small intestine after 72 hours. Results Compared with the normal group, the model group had a significant increase in diarrhea index (P<0.05); compared with the model group, the Tianshu group, the Dachangshu group, and the Tianshu+Dachangshu group had a significant reduction in diarrhea index (P<0.05); compared with the Tianshu group and the Dachangshu group, the Tianshu+Dachangshu group had a significant reduction in diarrhea index (P<0.05). Compared with the normal group, the model group had a significant reduction in the number of AQP8-positive cells (P<0.05) and significant increases in the numbers of VIP- and SP-positive cells in the small intestine (P<0.05); compared with the model group, the Tianshu+Dachangshu group had a significant increase in the number of AQP8-positive cells (P<0.05) and significant reductions in the numbers of VIP- and SP-positive cells in the small intestine (P<0.05). Conclusion Tianshu and Dachangshu have a synergistic effect in the treatment of diarrhea, and moxibustion exerts a therapeutic effect on diarrhea possibly by upregulating the expression of AQP8 and inhibiting the expression of VIP and SP.

    参考文献
    相似文献
    引证文献
引用本文

杨晓希,武小利,高 纺,吴生兵,周美启.不同穴位艾灸对腹泻模型小鼠腹泻指数及小肠AQP8、VIP及SP表达的影响[J].安徽中医药大学学报,2020,39(2):50-53

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2020-04-10