Abstract:Objective To investigate the effect of Bushen Antai Granule on VHL and HIF-1α in the decidual tissue of mice with recurrent spontaneous abortion (RSA) and its mechanism of action in reducing embryo loss rate. Methods Female CBA/J mice and male BALB/C mice were caged to establish a mouse model of normal pregnancy, and female CBA/J mice and male DBA/2 mice were caged to establish a mouse model of RSA. The mice were randomly divided into model group, progesterone group, and high-, middle-, and low-dose Bushen Antai Granule groups, with 10 mice in each group. On day 1 of pregnancy, the mice in the normal group and the model group were given distilled water by gavage, those in the progesterone group were given progesterone at a dose of 26 mg/(kg·d) by gavage,and those in the high-,middle-, and low-dose Bushen Antai Granule groups were given Bushen Antai Granule at a dose of 35.1, 11.7, and 3.9 g/(kg·d), respectively, by gavage. After 15 days of consecutive intervention, the mice were sacrificed and the samples were collected. ELISA was used to measure the serum levels of VHL and HIF-1α, and quantitative real-time PCR, immunohistochemistry, and Western blot were used to measure the mRNA and protein expression of VHL and HIF-1α in decidual tissue. Results Bushen Antai Granule significantly reduced the embryo loss rate of RSA mice (P<0.05), the serum level of VHL, and the mRNA expression of VHL in decidual tissue (P<0.05) and significantly increased the serum level of HIF-1α and the mRNA expression of HIF-1α in decidual tissue (P<0.05), in a dose-dependent manner (P<0.05). Conclusion Bushen Antai Granule can reduce the abortion rate of RSA mice, possibly by activating the VHL/HIF-1α signaling pathway.