表儿茶素对四氯化碳诱导肝纤维化大鼠的干预作用
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安徽省国际科技合作项目(1403062024);茶树生物资源与利用国家重点实验室开放基金项目(SKLTOF 20160107)


Intervention Effect of Epicatechin on Rats with Liver Fibrosis Induced by Carbon Tetrachloride
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    摘要:

    目的 研究表儿茶素对四氯化碳诱导大鼠肝纤维化的保护作用。方法 将80只雄性SD大鼠随机分为正常对照组、模型组、秋水仙碱组(0.1 mg/kg)、表儿茶素高剂量组(100 mg/kg)、表儿茶素低剂量组(25 mg/kg)。除正常对照组腹腔注射橄榄油外,其余各组均腹腔注射四氯化碳(CCl4) 建立肝纤维化模型。复制模型的同时开始给药,各给药组大鼠灌胃相应药物,正常对照组和模型组大鼠灌胃等容积生理盐水,每日1次,连续6周。末次给药后,称取肝湿质量,计算肝脏指数;采用微板法检测大鼠血清中天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)水平;苏木精-伊红染色和Masson胶原染色观察肝脏病理学变化;Western blot法检测肝脏中平滑肌肌动蛋白(alpha smooth muscle actin,α-SMA)、Ⅰ型胶原(typeⅠcollagen,collα1)蛋白的表达水平。结果 与模型组相比,表儿茶素能显著降低大鼠的肝脏指数和血清ALT、AST的含量;显著降低肝组织中α-SMA、collα1蛋白的表达;肝组织病理学指标明显改善。结论 表儿茶素对四氯化碳诱导的大鼠肝纤维化有一定的保护作用,其机制可能与抑制α-SMA、collα1的表达有关。

    Abstract:

    Objective To investigate the protective effect of epicatechin against liver fibrosis induced by carbon tetrachloride (CCl4) in rats. Methods A total of 80 male Sprague-Dawley rats were randomly divided into normal control group, model group, colchicine group (0.1 mg/kg), high-dose epicatechin group (100 mg/kg), and low-dose epicatechin group (25 mg/kg). The rats in the normal control group were given intraperitoneal injection of olive oil, and those in the other groups were given intraperitoneal injection of CCl4 to establish a model of liver fibrosis. The rats in the administration groups were given the corresponding drug by gavage since the first day of modeling, and those in the normal control group and the model group were given an equal volume of normal saline by gavage, once a day for 6 consecutive weeks. After the last administration, hepatic wet weight was measured and liver index was calculated; the microplate method was used to measure the serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT); hematoxylin and eosin staining and Masson collagen staining were used to observe liver pathological changes;Western blot was used to measure the protein expression of alpha smooth muscle actin (α-SMA) and collagen α1 (Collα1) in the liver. Results Compared with the model group, the epicatechin groups had significant reductions in liver index, serum levels of ALT and AST, and protein expression of α-SMA and Collα1 in the liver, as well as significant improvements in liver pathological indices. Conclusion Epicatechin has a certain protective effect on CCl4-induced liver fibrosis in rats, possibly by inhibiting the expression of α-SMA and Collα1.

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王玉涵,展 凡,程 卉,郑 凤,谢忠稳,李庆林.表儿茶素对四氯化碳诱导肝纤维化大鼠的干预作用[J].安徽中医药大学学报,2020,39(1):50-54

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  • 在线发布日期: 2020-01-17