Comparison of the Content of Five Coumarins in Sulfur-free and Sulfur-smoked Radix Angelicae Dahuricae by Quantitative Analysis of Multi-components by Single Marker
Objective To investigate the difference in the content of five coumarins between sulfur-free and sulfur-smoked Radix Angelicae Dahuricae based on the quantitative analysis of multi-components by single marker (QAMS), and to evaluate the accuracy and feasibility of this method. Methods Imperatorin was used as the internal reference substance and its relative correction factors with the other four components were established. The content of the other four coumarins was calculated and compared with the results calculated by the external standard method to verify the feasibility of the method. Results The RSD of the relative correction factor was within 3.0 % in different experimental conditions and there was no significant difference between the results of QAMS and the external standard method. Conclusion QAMS can be used for simultaneous determination of the content of five coumarins in sulfur-free and sulfur-smoked Radix Angelicae Dahuricae.