安徽“粉性葛”与“柴性葛”中6种异黄酮类化合物含量比较
Comparison of the Content of Six Isoflavones in the Mealy-like Roots and the Wooden-like Roots of Pueraria lobata from Anhui Province, China
  
DOI:
中文关键词:  葛根  粉性葛  柴性葛  异黄酮  高效液相色谱法
英文关键词:Pueraria lobate  Mealy-like root  Wooden-like root  Isoflavone  High-performance liquid chromatography
基金项目:安徽省高校自然科学研究重点项目(KJ2017A296,KJ2019A1235);安徽省高校优秀青年骨干人才国内访学研修项目(gxgnfx2018094);安徽省高等学校省级质量工程项目(2018jxtd026)
作者单位
刘 毅,尹旻臻,徐 涛,段海燕,程铭恩 1. 安庆医药高等专科学校安徽 安庆 2460522.安徽中医药大学药学院安徽 合肥 230012 
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中文摘要:
      目的 采用高效液相色谱法同时测定安徽省“粉性葛”与“柴性葛”中6种异黄酮化合物含量。方法 采用Agilent Zorbax XDB-C18色谱柱(250 mm×4.6 mm,3.5 μm),流动相为甲醇(A)-0.2%醋酸水(B),0.8 mL/min梯度洗脱,检测波长250 nm,柱温30 ℃。结果 26批葛根样品中6种异黄酮化合物在48 min内完全分离且线性关系良好(r>0.999),3′-羟基葛根素、葛根素、3′-甲氧基葛根素、葛根素芹菜糖苷、大豆苷、大豆苷元的平均回收率分别为98.6%、103.4%、98.5%、102.1%、105.9%、100.7%(n=6)。聚类分析和主成分分析结果表明,“粉性葛”与“柴性葛”分别聚为两类。结论 “粉性葛”与“柴性葛”中异黄酮类化合物的含量有差异,其中,“粉性葛”中葛根素含量较“柴性葛”偏低。
英文摘要:
      Objective To simultaneously determine the content of six isoflavones in the mealy-like roots and the wooden-like roots of Pueraria lobata from Anhui Province, China using high-performance liquid chromatography (HPLC). Methods HPLC was performed on an Agilent Zorbax XDB-C18 column (250 mm × 4.6 mm, 3.5 μm) with a mobile phase of methanol (A)-0.2% acetic acid (B) for gradient elution, at a flow rate of 0.8 mL/min, a detection wavelength of 250 nm, and a column temperature of 30 ℃. Results In the 26 batches of Pueraria lobata roots, the six isoflavones were completely separated within 48 minutes and had a good linear relationship (r>0.999). The average recovery rates of 3′-hydroxy puerarin, puerarin, 3′-methoxy puerarin, puerarin celery glycoside, daidzin, and daidzein in Pueraria lobata roots were 98.6%, 103.4%, 98.5%, 102.1%, 105.9%, and 100.7%, respectively (n=6). The results of the cluster analysis and the principal component analysis showed that the mealy-like roots and the wooden-like roots were divided into two categories. Conclusion There is a great difference in the content of isoflavones between the mealy-like roots and the wooden-like roots of Pueraria lobate, and the mealy-like roots have a lower content of puerarin than the wooden-like roots.
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