芍药汤对溃疡性结肠炎大鼠结肠组织机械屏障及细胞因子的影响
Effects of Shaoyao Decoction on Mechanical Barrier and Cytokines in the Colon Tissue of Rats with Ulcerative Colitis
  
DOI:
中文关键词:  溃疡性结肠炎  芍药汤  炎症反应  肠黏膜机械屏障
英文关键词:Ulcerative colitis  Shaoyao Decoction  Inflammatory response  Colonic mucosal mechanical barrier
基金项目:湖南省中医药科研计划重点项目(201804);湖南省卫生计生委科研计划项目(C20180627);湖南中医药大学校级科研基金项目(ZYYDX201733)
作者单位
陈大光,曹 晖,李 令,吴东升,刘震宇,陈鸿飞,田 娜 湖南中医药大学湖南 长沙 410007 
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中文摘要:
      目的 基于结肠组织机械屏障和炎症细胞因子探讨芍药汤治疗溃疡性结肠炎的作用机制。〖JP〗方法 将SD大鼠随机分为空白对照组、模型组、美沙拉嗪组、芍药汤组。采用TNBS/乙醇复制溃疡性结肠炎大鼠模型,各组分别使用生理盐水、美沙拉嗪、芍药汤进行干预。比较各组大鼠一般情况、疾病活动指数(disease activity index, DAI)评分,观察结肠组织形态学变化和结肠组织紧密连接,采用ELISA法检测血清肿瘤坏死因子-α(tumor necrosis factor alpha, TNF-α)、白细胞介素-4(interleukin-4, IL-4)和内毒素(endotoxin, ET)表达水平。结果 与模型组比较,芍药汤组大鼠体质量逐渐回升,腹泻及便血减少;与模型组比较,美沙拉嗪组、芍药汤组DAI评分,组织形态学评分,IL-4、ET、TNF-α表达水平显著降低,差异均有统计学意义(P<0.05)。透射电镜下模型组大鼠结肠上皮组织紧密连接明显破坏,美沙拉嗪组、芍药汤组大鼠结肠上皮组织紧密连接破坏情况较模型组轻。结论 芍药汤可能是通过修复肠上皮组织紧密连接,减轻炎症反应,保护肠黏膜机械屏障而发挥治疗溃疡性结肠炎的作用。
英文摘要:
      Objective To investigate the mechanism of action of Shaoyao Decoction (SYD) in the treatment of ulcerative colitis based on its effects on the mechanical barrier and inflammatory cytokines of colon tissue. Methods Sprague-Dawley rats were randomly divided into control group, model group, mesalazine group, and SYD group. A rat model of ulcerative colitis was established by 2,4,6-trinitrobenzenesulfonic acid/ethanol, and the model group, mesalazine group, and SYD group were treated with normal saline, mesalazine, and SYD, respectively. General condition and disease activity index (DAI) score were compared between groups. The morphological changes in colon tissue and colonic epithelial tight junction were evaluated. The expression levels of serum interleukin-4 (IL-4), endotoxin (ET), and tumor necrosis factor-α (TNF-α) were determined by ELISA. Results Compared with the model group, the SYD group showed gradually increased body weight and reduced occurrence of diarrhea and hematochezia, and the mesalazine group and SYD group had significantly decreased DAI score, histomorphological score, IL-4, ET, and TNF-α (P<0.05). Under the transmission electron microscope, the model group showed obvious colonic epithelial tight junction injury, which was milder in the mesalazine group and the SYD group. Conclusion SYD may play a role in the treatment of ulcerative colitis by repairing the damaged colonic epithelial tight junction, reducing inflammatory response, and protecting the colonic mucosal mechanical barrier.
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