W/O型黄芩苷纳米乳的制备及质量评价
Preparation and Quality Assessment of W/O Baicalin Nanoemulsion
  
DOI:
中文关键词:  黄芩苷  W/O型黄芩苷纳米乳  质量评价  高效液相色谱法  稳定性
英文关键词:Baicalin  W/O baicalin nanoemulsion  Quality assessment  High-performance liquid chromatography  Stability
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作者单位
郑转弟,周 安,吴鸿飞 1.安徽中医药大学药学院安徽 合肥 2300122.安徽中医药大学科研实验中心安徽 合肥 230038 
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中文摘要:
      目的 制备W/O型黄芩苷纳米乳并对其质量进行评价。方法 通过优化处方工艺制得W/O型黄芩苷纳米乳,并对其外观、粒径、载药量及稳定性进行考察,采用高效液相色谱法测定纳米乳中黄芩苷的含量,检测波长为280 nm。结果 W/O型黄芩苷纳米乳为均一透明的液体,平均粒径为(63.40±1.10)nm,载药量为9.5 mg/mL。黄芩苷在12.40~310.00 μg/mL浓度范围内与峰面积线性关系良好,r=0.999 7。高、中、低质量浓度的黄芩苷供试品溶液平均加样回收率分别为99.88%、99.01%、100.31%,RSD均小于0.87%。黄芩苷纳米乳在常温和低温条件下贮存30 d,含量无明显变化。结论 制备的W/O型黄芩苷纳米乳质量稳定可靠,建立的高效液相色谱法操作简便、快捷、重复性好,可用于测定纳米乳中黄芩苷的含量。
英文摘要:
      Objective To prepare water-in-oil (W/O) baicalin nanoemulsion and assess its quality. Methods W/O baicalin nanoemulsion was prepared by the optimized formulation process. The appearance, particle size, drug loading, and stability of W/O baicalin nanoemulsion were investigated. High-performance liquid chromatography (HPLC) was performed at a detection wavelength of 280 nm to determine the content of baicalin in nanoemulsion. Results W/O baicalin nanoemulsion was uniform transparent liquid, with an average particle size of (63.40±1.097) nm and a drug loading of 9.5 mg/mL. The concentration of baicalin showed a good linear relationship with peak area within 12.40-310.00 μg/mL (r=0.999 7). The average recovery rates of high-, middle-, and low-concentration baicalin sample solutions were 99.88%, 99.01%, and 100.31%, respectively, with relative standard deviations of <0.87%. No significant change in the content of baicalin was observed after baicalin nanoemulsion was stored at normal or low temperature for 30 days. Conclusion The prepared W/O baicalin nanoemulsion has stable and reliable quality. The established HPLC method is simple, efficient, and repeatable, and can be used to determine baicalin in nanoemulsion.
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