三七破壁饮片质量分析
A Quality Analysis of Panax notoginseng Ultrafine Decoction Pieces
  
DOI:
中文关键词:  三七破壁饮片  显微鉴定  薄层鉴别  指纹图谱  粒径分布  含量测定
英文关键词:Panax notoginseng ultrafine decoction pieces  Microscopic identification  Thin-layer chromatography  Fingerprint  Particle size distribution  Content determination
基金项目:安徽中医药大学自然科学研究项目(2017fyyb007)
作者单位
梁 笑,徐国兵,钱珊珊,桂双英,王举涛,葛 鼎,剧梦真,鲍炳娟 1.安徽中医药大学药学院安徽 合肥 2300122.安徽省食品药品检验研究院安徽 合肥 2300513.安徽中医药大学第二附属医院药学部安徽 合肥 2300614.安徽省中医药科学院药物制剂研究所安徽 合肥 2300125.安徽道地中药材品质提升协同创新中心安徽 合肥 230012 
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中文摘要:
      目的 建立三七破壁饮片的质量控制方法。方法 参考2015年版《中华人民共和国药典》相关检测方法,对10批三七破壁饮片的性状、显微特征、薄层鉴别、指纹图谱及其水分、灰分、浸出物、重金属、粒径分布以及人参皂苷Rb1、人参皂苷Rg1、三七皂苷R1的含量等进行检测,建立三七破壁饮片的质量控制方法。结果 三七破壁饮片为灰褐色至灰黄色的颗粒,气微,味苦回甜。显微镜下可见淀粉粒甚多,单粒圆形、半圆形或圆多角形,直径4~30 μm,脂道碎片含黄色分泌物,导管碎片较少。薄层鉴别显示,10批样品在与对照品色谱相应的位置上,均显示相同颜色的斑点。水分范围为5.05%~9.82%,总灰分范围为2.96%~3.20%,酸不溶性灰分范围为0.52%~1.19%,浸出物含量范围为21.03%~27.78%,每0.01 g样品中未破壁细胞数为46~81。指纹图谱显示,各批次三七破壁饮片的色谱图相似度为0.879~0.996。粒径分析显示,各样品D90为34.09~38.93 μm。10批样品中人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1总含量为6.9%~8.3%。结论 建立的方法可用于三七破壁饮片的质量控制。
英文摘要:
      Objective To establish the quality control method for Panax notoginseng ultrafine decoction pieces. Methods With reference to related methods in the 2015 edition of Pharmacopoeia of The People's Republic of China, 10 batches of Panax notoginseng ultrafine decoction pieces were analyzed in terms of properties, microscopic features, thin-layer chromatography (TLC) identification, fingerprint, water content, ash content, extract, heavy metals, particle size distribution, and content of ginsenoside Rb1, ginsenoside Rg1, and notoginsenoside R1, so as to establish the quality control method for Panax notoginseng ultrafine decoction pieces. Results Panax notoginseng ultrafine decoction pieces were taupe to gray-yellow particles with a touch of smell, a bitter taste, and a sweet aftertaste. Microscopic observation showed a large number of starch grains with a round, semi-circular, or polygonal shape and a diameter of 4-30 μm, yellow secretions in lipid debris, and a small amount of ductal debris. TLC identification showed that the 10 batches of samples and the reference samples had spots with the same color at the corresponding position of the chromatograph. The ranges of water content, total ash, acid-insoluble ash, and extract were 5.05%-9.82%, 2.96%-3.20%, 0.52%-1.19%, and 21.03%-27.78%, respectively. The number of unbroken cells in each 0.01 g sample ranged from 46 to 81. The fingerprint analysis showed a similarity of 0.879-0.996 between the 10 batches of Panax notoginseng ultrafine decoction pieces. Particle size analysis showed that D90 of these samples ranged from 34.09 to 38.93 μm. The total content of ginsenoside Rg1, ginsenoside Rb1, and notoginsenoside R1 in the 10 batches of samples ranged from 6.9% to 8.3%. Conclusion The method established in this study can be used for the quality control of Panax notoginseng ultrafine decoction pieces.
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