平喘宁对寒性哮喘大鼠PI3K/Akt信号通路相关信号分子表达的影响
Effect of Pingchuanning on the Expression of Signaling Molecules Associated with the PI3K/Akt Signaling Pathway in Rats with Cold-type Asthma
  
DOI:
中文关键词:  哮喘  平喘宁  Bad  FKHR  蛋白激酶C  气道重塑
英文关键词:Asthma  Pingchuanning  Bad  Forkhead box protein O1  Protein kinase C  Airway remodeling
基金项目:安徽省高校自然科学研究重点项目(KJ2018A0281)
作者单位
孟 芝,方向明 1.安徽中医药大学安徽 合肥 2300122.中药复方安徽省重点实验室安徽 合肥 230012 
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中文摘要:
      目的 探讨平喘宁调节PI3K/Akt信号通路相关蛋白干预寒性哮喘大鼠的作用机制。方法 随机将105只SD健康雄性大鼠分为正常组、模型组、平喘宁高剂量组、平喘宁中剂量组、平喘宁低剂量组、桂龙咳喘宁组、地塞米松组,每组15只。除正常组外其余各组以卵蛋白进行致敏,并予以哮喘诱导及寒冷刺激。模型复制后,平喘宁高、中、低剂量组(14.6、7.3、3.7 g/kg)和地塞米松组(0.4 mg/kg)、桂龙咳喘宁组(0.4 g/kg)按相应剂量灌胃治疗,正常组和模型组用生理盐水代替。取肺组织,用苏木素-伊红染色法检测病理变化,实时荧光定量PCR法检测Bad、叉头蛋白O1(forkhead box protein O1,FKHR)、蛋白激酶C(protein kinase C,PKC) mRNA的表达水平。结果 与正常组比较,模型组大鼠肺组织管壁明显增厚,支气管结构明显紊乱,有炎症细胞浸润等现象;与模型组比较,药物治疗组大鼠肺组织的病理变化均有所改善。与正常组比较,模型组大鼠肺组织Bad mRNA、FKHR mRNA、PKC mRNA表达差异均有统计学意义(P<0.05);与模型组比较,药物组Bad mRNA、FKHR mRNA、PKC mRNA表达差异均有统计学意义(P<0.05),并且平喘宁对PKC、Bad、FKHR mRNA表达的效应具有剂量依赖性,平喘宁高剂量组作用优于其他药物组。结论 平喘宁可以通过调控PI3K/Akt信号传导通路中PKC、FKHR、Bad的表达水平,减缓寒哮大鼠肺组织气道重塑,减轻炎症反应,改善哮喘症状。
英文摘要:
      Objective To investigate the mechanism of action of Pingchuanning in the regulation of the proteins associated with the PI3K/Akt signaling pathway and the intervention of rats with cold-type asthma.Methods A total of 105 healthy male Sprague-Dawley rats were randomly divided into normal group, model group, high-, middle-, and low-dose Pingchuanning groups,Guilong Kechuanning group, and dexamethasone group,with 15 rats in each group.All rats except those in the normal group were given ovalbumin for sensitization,as well as induction of asthma and cold stimulation. After the model was established, the rats in the high-, middle-, and low-dose Pingchuanning groups were given Pingchuanning by gavage at a dose of 14.6, 7.3, and 3.7 g/kg, respectively, those in the dexamethasone group were given dexamethasone by gavage at a dose of 0.4 mg/kg, and those in the Guilong Kechuanning group were given Guilong Kechuanning by gavage at a dose of 0.4 g/kg. The rats in the normal group and the model group were given normal saline. After lung tissue samples were collected, HE staining was used to observe pathological changes, and quantitative real-time PCR was used to measure the mRNA expression of Bad, forkhead box protein O1 (FKHR), and protein kinase C (PKC). Results Compared with the normal group, the model group had marked wall thickening and disorganized bronchial structure in lung tissue, with the presence of inflammatory cell infiltration; compared with the model group, all drug treatment groups had improvements in the pathological changes of lung tissue. There were significant differences in the mRNA expression of Bad, FKHR, and PKC in lung tissue between the normal group and the model group (P<0.05), as well as between the model group and the drug treatment groups (P<0.05). Pingchuanning acted on the mRNA expression of PKC, Bad, and FKHR in a dose-dependent manner, and the high-dose Pingchuanning group had better improvements than the other drug treatment groups. Conclusion By regulating the expression of PKC, FKHR, and Bad in the PI3K/Akt signaling pathway, Pingchuanning can reduce airway remodeling, alleviate inflammatory response, and improve the symptoms of asthma in rats with cold-type asthma.
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