桃红四物汤对脑微血管内皮细胞-PC12细胞共培养体系缺糖缺氧再灌注损伤模型的保护作用
Protective Effect of Taohong Siwu Decoction Against Reperfusion Injury Due to Oxygen-Glucose Deprivation in Brain Microvascular Endothelial Cell-PC12 Cell Co-culture System
  
DOI:
中文关键词:  桃红四物汤  缺糖缺氧  再灌注损伤  脑微血管内皮细胞  PC12细胞  共培养  抗氧化作用
英文关键词:Taohong Siwu Decoction  Oxygen-glucose deprivation  Reperfusion injury  Brain microvascular endothelial cell  PC12 cell  Co-culture  Antioxidant effect
基金项目:国家自然科学基金项目(81503291);安徽省重点研究与开发计划项目(1704a0802141)
作者单位
陈芳芳,韩 岚,彭代银,汪蒙蒙,胡寿山,夏文文 安徽中医药大学药学院 安徽省中药复方重点实验室安徽 合肥 230012 
摘要点击次数: 1200
全文下载次数: 1668
中文摘要:
      目的 观察桃红四物汤对脑微血管内皮细胞-PC12细胞共培养体系缺糖缺氧再灌注损伤模型的保护作用。方法 采用氧-葡萄糖剥夺再恢复的方法诱导脑微血管内皮细胞(brain microvascular endothelial cells, BMECs)和PC12细胞共培养体系的缺糖缺氧再灌注损伤模型,并通过流式细胞仪检测PC12细胞活性氧(reactive oxygen species,ROS)水平,酶联免疫吸附法检测丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性,采用Western Blot法检测Bcl-2、Bax和缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)的表达水平。结果 桃红四物汤促进PC12细胞的活力,抑制细胞凋亡和ROS的产生,抑制MDA的活性,增强SOD和GSH-Px的活性,上调HIF-1α和Bcl-2的表达并下调Bax的表达。结论 桃红四物汤对脑缺血再灌注损伤的保护作用可能与抗氧化作用有关。
英文摘要:
      Objective To investigate the protective effect of Taohong Siwu Decoction against reperfusion injury due to oxygen-glucose deprivation in brain microvascular endothelial cell (BMEC)-PC12 cell co-culture system. Methods Oxygen-glucose deprivation/recovery was performed to induce reperfusion injury due to oxygen-glucose deprivation in the BMEC-PC12 cell co-culture system. Flow cytometry was used to measure reactive oxygen species (ROS) in PC12 cells; enzyme-linked immunosorbent assay was used to measure the activities of malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px); Western blot was used to measure the expression of Bcl-2, Bax, and hypoxia-inducible factor-1α (HIF-1α). Results Taohong Siwu Decoction promoted the viability of PC12 cells, inhibited cell apoptosis, reduced the production of ROS, and enhanced the activities of SOD and GSH-Px. In addition, it upregulated the expression of HIF-1α and Bcl-2 and downregulated the expression of Bax. Conclusion Taohong Siwu Decoction exerts a protective effect against cerebral ischemia/reperfusion injury via its antioxidant effect.
查看全文  查看/发表评论  下载PDF阅读器
关闭