电针预处理不同原络配穴对急性心肌缺血再灌注损伤大鼠心肌组织TNF-α、COX-2、ICAM-1蛋白表达的影响
Effect of Electroacupuncture Pretreatment at Yuan-Source and Luo-Collateral Acupoints on the Expression of Tumor Necrosis Factor-α, Cyclooxygenase-2, and Intercellular Adhesion Molecule-1 in Myocardial Tissue in Rats with Acute Myocardial Ischemia-Reperfusion Injury
  
DOI:
中文关键词:  急性心肌缺血再灌注  原络配穴  肿瘤坏死因子-α  环氧合酶-2  细胞间黏附分子-1
英文关键词:Acute myocardial ischemia-reperfusion  Combinations of Yuan-source and Luo-collateral acupoints  Tumor necrosis factor-α  Cyclooxygenase-2  Intercellular adhesion molecule-1
基金项目:国家自然科学基金项目(81774414);安徽省高校优秀青年人才支持计划重点项目(gxyqZD2016134);安徽高校科研创新平台建设项目(2015TD033)
作者单位
邵雪芳,蔡荣林,曹 奕,吴生兵,吴子建,王璐璐,刘 磊,郑良玉,李小贾 1.安徽中医药大学研究生院安徽 合肥 2300122.安徽中医药大学针灸推拿学院安徽 合肥 2300123.安徽中医药大学第二附属医院安徽 合肥 2300614.安徽中医药大学科研实验中心安徽 合肥 230038 
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中文摘要:
      目的 观察电针预处理不同原络配穴对急性心肌缺血再灌注损伤大鼠心肌炎症因子表达的影响,初步探讨其保护心肌的作用机制。方法 采用冠状动脉左前降支结扎再灌注法复制急性心肌缺血再灌注损伤模型,将模型大鼠随机分为模型对照组、神门支正组、神门通里组、大陵内关组、大陵外关组,每组6只,另取6只大鼠设为假手术组。电针预处理组分别选取神门、支正、通里、内关、大陵和外关穴位,用电针仪进行电针刺激,刺激电压1 V,频率为2 Hz,每日1次,每次20 min。模型复制前共刺激14 d。假手术组、模型组不给予电针刺激。监测大鼠心电图,分析ST段位移情况;采用免疫组织化学法检测心肌组织肿瘤坏死因子-α(tumor necrosis factor-alpha, TNF-α)、细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)、环氧合酶-2(cyclooxygenase-2, COX-2)表达水平。采用Wester blot 法检测心肌组织COX-2蛋白相对表达水平。结果 电针预处理不同原络配穴均可显著降低急性心肌缺血再灌注损伤模型大鼠升高的ST段电压(P<0.05),以神门支正组的效应最显著。免疫组织化学法检测结果显示,电针预处理不同原络配穴均可显著降低模型大鼠心肌组织TNF-α、COX-2、ICAM-1表达水平(P<0.05), 以神门支正组的效应最显著。Western blot法检测结果显示,各组心肌组织COX-2蛋白表达水平与免疫组织化学法检测结果相一致。结论 电针预处理减轻急性心肌缺血再灌注损伤的机制与调节心肌组织TNF-α、COX-2、ICAM-1表达水平有关,电针神门、支正穴的效应最为显著。
英文摘要:
      Objective To investigate the effect of electroacupuncture with different combinations of Yuan-source and Luo-collateral acupoints on the expression of inflammatory factors in myocardial tissue in rats with acute myocardial ischemia-reperfusion injury and the mechanism of action of electroacupuncture in protecting myocardium. Methods A rat model of acute myocardial ischemia-reperfusion injury was established by ligation and reperfusion of the left anterior descending coronary artery. The model rats were randomly divided into model control group, Shenmen+Zhizheng electroacupuncture group, Shenmen+Tongli electroacupuncture group, Daling+Neiguan electroacupuncture group, and Daling+Waiguan electroacupuncture group, with 6 rats in each group. Another 6 rats were selected as sham-operation group. The rats in the electroacupuncture groups were given electroacupuncture pretreatment with different combinations of Shenmen, Zhizheng, Tongli, Neiguan, Daling, and Waiguan acupoints 20 minutes once daily, and an electroacupuncture apparatus was used for stimulation at a voltage of 1 V and a frequency of 2 Hz. Electroacupuncture stimulation was given for 14 days before modeling, and the rats in the sham-operation group and the model group were not given electroacupuncture stimulation. The rats were given electrocardiographic monitoring to observe the displacement of ST segment. Immunohistochemistry was used to measure the expression of tumor necrosis factor-α (TNF-α), intercellular adhesion molecule-1 (ICAM-1), and cyclooxygenase 2 (COX-2) in myocardial tissue. Western blot was used to measure the relative protein expression of COX-2 in myocardial tissue. Results Electroacupuncture pretreatment with different combinations of Yuan-source and Luo-collateral acupoints significantly reduced the elevated voltage of ST segment in rats with acute myocardial ischemia-reperfusion injury (P<0.05), and the greatest reduction was observed in the Shenmen+Zhizheng electroacupuncture group. Immunohistochemistry showed that electroacupuncture pretreatment with different combinations of Yuan-source and Luo-collateral acupoints significantly reduced the expression of TNF-α, COX-2, and ICAM-1 in myocardial tissue in model rats (P<0.05), and the greatest reduction was observed in the Shenmen+Zhizheng electroacupuncture group. Western blot showed that the protein expression of COX-2 in myocardial tissue in each group was consistent with the results of immunohistochemistry. Conclusion Electroacupuncture pretreatment can alleviate acute myocardial ischemia-reperfusion injury by regulating the expression of TNF-α, COX-2, and ICAM-1 in myocardial tissue, and electroacupuncture at Shenmen and Zhizheng acupoints has the best effect.
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