桃红四物汤中6种指标性成分的含量测定
Content Determination of Six Index Components in Taohong Siwu Decoction
  
DOI:
中文关键词:  桃红四物汤  指标性成分  含量测定  超高效液相色谱
英文关键词:Taohong Siwu Decoction  Index component  Content determination  Ultra- performance liquid chromatography
基金项目:国家自然科学基金项目(81503291,81473387);安徽省重点研究与开发计划项目(1704a0802141)
作者单位
王继陈,韩 岚,张艳艳,彭代银 1.安徽中医药大学药学院安徽 合肥 2300122.中药复方安徽省重点实验室安徽 合肥 2300123.安徽医学高等专科学校安徽 合肥 230601 
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中文摘要:
      目的 建立超高效液相色谱法同时测定桃红四物汤中6种指标性成分(羟基红花黄色素A、苦杏仁苷、芍药苷、阿魏酸、毛蕊花糖苷、藁本内酯)的含量。方法 采用Agilent Technologies 1290 Infinity超高效液相色谱系统、DAD检测器。色谱柱为Waters ACQUITY UPLC BEH C18柱(2.1 mm×100 mm,1.7 μm),流动相A为0.05%乙酸水(含7.84 mmol/L乙酸铵),B为甲醇溶液,采用梯度洗脱程序和多波长融合检测方法,流速0.2 μL/min,进样量2 μL,柱温25 ℃。结果 6种指标性成分的线性、分离度、稳定性、重复性与精密度良好;该方法能够快速、稳定地定量羟基红花黄色素A、苦杏仁苷、芍药苷、阿魏酸、毛蕊花糖苷、藁本内酯。结论 该方法稳定、可靠、重复性高,可适用于桃红四物汤的质量控制。
英文摘要:
      Objective To establish an ultra- performance liquid chromatography (UPLC) method for the simultaneous determination of six index components in Taohong Siwu Decoction, i.e., hydroxysafflor yellow A, amygdalin, paeoniflorin, ferulic acid, verbascoside, and ligustilide. Methods The Agilent Technologies 1290 Infinity UPLC system and a DAD detector were used for content determination. UPLC was performed on A Waters ACQUITY UPLC BEH C18 column (2.1 mm×100 mm, 1.7 μm), with a mobile phase A of 0.05% acetic acid water (containing 7.84 mmol/L ammonium acetate) and a mobile phase B of methanol solution for gradient elution and multi- wavelength detection, at a flow rate of 0.2 μL/min, a sample size of 2 μL, and a column temperature of 25 ℃. Results All 6 components had good linearity, resolution, stability, reproducibility, and precision. This method could be used for rapid and stable quantitative determination of hydroxysafflor yellow A, amygdalin, paeoniflorin, ferulic acid, verbascoside, and ligustilide. Conclusion The method is stable and reliable with high reproducibility and can be used for the quality control of Taohong Siwu Decoction.
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