Abstract:Objective To establish an ultra- performance liquid chromatography (UPLC) method for the simultaneous determination of six index components in Taohong Siwu Decoction, i.e., hydroxysafflor yellow A, amygdalin, paeoniflorin, ferulic acid, verbascoside, and ligustilide. Methods The Agilent Technologies 1290 Infinity UPLC system and a DAD detector were used for content determination. UPLC was performed on A Waters ACQUITY UPLC BEH C18 column (2.1 mm×100 mm, 1.7 μm), with a mobile phase A of 0.05% acetic acid water (containing 7.84 mmol/L ammonium acetate) and a mobile phase B of methanol solution for gradient elution and multi- wavelength detection, at a flow rate of 0.2 μL/min, a sample size of 2 μL, and a column temperature of 25 ℃. Results All 6 components had good linearity, resolution, stability, reproducibility, and precision. This method could be used for rapid and stable quantitative determination of hydroxysafflor yellow A, amygdalin, paeoniflorin, ferulic acid, verbascoside, and ligustilide. Conclusion The method is stable and reliable with high reproducibility and can be used for the quality control of Taohong Siwu Decoction.