耳针对血管性痴呆大鼠认知功能及海马神经细胞的影响
Effect of Ear Acupuncture on Cognitive Function and Hippocampal Neurons in Rats with Vascular Dementia
  
DOI:
中文关键词:  血管性痴呆  认知功能  耳针  海马
英文关键词:Vascular dementia  Cognitive function  Auricular acupuncture  Hippocampus
基金项目:国家自然科学基金面上项目(8157151194);安徽省科技计划项目(11070403041)
作者单位
乔晓迪,张媛媛,张庆萍,井 杰 1.安徽中医药大学研究生院安徽 合肥 2300122.安徽中医药大学第一附属医院安徽 合肥 2300313.安徽中医药大学针灸推拿学院安徽 合肥 2300124.杭州市余杭区第五人民医院浙江 杭州 311100 
摘要点击次数: 1256
全文下载次数: 1837
中文摘要:
      目的 动态观察耳针对血管性痴呆(vascular dementia,VD)大鼠认知功能及海马神经细胞的影响。方法 从60只健康雄性SD大鼠中随机选取10只为正常组,其余采用四血管阻断法复制VD大鼠模型,然后随机分为模型组、耳针组、西药组,每组10只。于模型复制后第4天,治疗第30、60天时,采用跳台实验检测各组大鼠的学习记忆成绩,并进行神经行为学评分;于模型复制后第4天、治疗第60天时,光镜下观察各组大鼠海马CA1区神经细胞形态。结果 模型复制后第4天,与正常组比较,模型组大鼠学习记忆成绩、神经行为学评分及海马CA1区锥体存活细胞数差异均有统计学意义(P<0.05);耳针治疗30、60 d后,与模型组比较,耳针组及西药组大鼠学习记忆成绩、神经行为学评分差异均有统计学意义(P<0.05),耳针组与西药组比较,差异无统计学意义(P>0.05);治疗60 d后,耳针组、西药组大鼠海马CA1区锥体细胞存活数与模型组比较,差异均有统计学意义(P<0.05);耳针组与西药组相比,差异无统计学意义(P>0.05)。结论 耳针可以提高VD大鼠的学习记忆能力,降低神经行为学评分;耳针可改善变性的海马CA1神经细胞,促进其修复与再生,从而提高VD大鼠学习记忆功能,这可能是临床耳针治疗VD有效作用的可能机制。
英文摘要:
      Objective To investigate the effect of ear acupuncture on cognitive function and hippocampal neurons in rats with vascular dementia (VD). Methods A total of 60 healthy male Sprague- Dawley rats were selected, among which 10 rats were enrolled as normal group and the remaining were treated with four- vessel occlusion to establish a model of VD and were then randomly divided into model group, auricular acupuncture group, and Western medicine group, with 10 rats in each group. On day 4 after modeling and on days 30 and 60 of treatment, the step- down test was performed to evaluate learning and memory performance and determine neurobehavioral score; on day 4 after modeling and on day 60 of treatment, the morphology of hippocampal CA1 neurons was observed under a light microscope. Results On day 4 after modeling, there were significant differences between the normal group and the model group in learning and memory performance, neurobehavioral score, and the number of viable pyramidal cells in the hippocampal CA1 region (P<0.05). On days 30 and 60 of ear acupuncture therapy, there were significant differences in learning and memory performance and neurobehavioral score between the auricular acupuncture/Western medicine groups and the model group (P<0.05), while there were no significant differences between the auricular acupuncture group and the Western medicine group (P>0.05). After 60 days of treatment, there were significant differences in these indices between the auricular acupuncture/Western medicine groups and the model group (P<0.05), while there were no significant differences between the auricular acupuncture group and the Western medicine group (P>0.05). Conclusion Auricular acupuncture can improve the learning and memory ability of VD rats and reduce their neurobehavioral score. It can also improve the degenerative hippocampal CA1 neurons, promote their recovery and regeneration, and thus improve the learning and memory ability of VD rats, which might be the possible mechanism of auricular acupuncture in the treatment of VD in clinical practice.
查看全文  查看/发表评论  下载PDF阅读器
关闭