绿萼梅药材超高效液相色谱指纹图谱研究
A Study of Fingerprints of Mume Flos by Ultra-performance Liquid Chromatography
  
DOI:
中文关键词:  绿萼梅  超高效液相色谱  指纹图谱
英文关键词:Mume Flos  Ultra-performance liquid chromatography  Fingerprint
基金项目:安徽省高等学校自然科学研究重点项目(KJ2017A295);安徽省科技攻关项目(1501051174);新安医学教育部重点实验室开放基金项目(2018xayx09)
作者单位
王灿灿,杨 沫,吴德玲,张 伟,金传山 1.安徽中医药大学药学院安徽 合肥 2300122.新安医学教育部重点实验室安徽 合肥 2300383.中药饮片制造新技术安徽省重点实验室安徽 合肥 230012 
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中文摘要:
      目的 采用超高效液相色谱法(ultra performance liquid chromatography,UPLC)建立绿萼梅药材的指纹图谱,为有效控制绿萼梅药材的质量奠定基础。方法 采用CORTECS C18色谱柱(2.1 mm×100 mm,1.6 μm),以乙腈(A)-0.1%甲酸水(B)溶液为流动相进行梯度洗脱,进样量为1 μL,流速为0.3 mL/min,检测波长260 nm。结果 通过相似度分析确定19个色谱峰构成绿萼梅药材指纹图谱的特征峰。结论 采用UPLC方法建立的指纹图谱具有精密度、稳定性、重复性良好的特点,可为绿萼梅化学成分的进一步提取分离和药效物质基础的研究提供科学依据。
英文摘要:
      Objective To establish the fingerprints of Mume Flos by ultra-performance liquid chromatography (UPLC), and to provide a basis for the effective control of quality of Mume Flos. Methods UPLC was performed on a CORTECS C18 column (2.1 mm × 100 mm, 1.6 mm) with a mobile phase of acetonitrile (A)-0.1% formic acid (B) solution for gradient elution at a sample size was of 1 μL, a flow rate of 0.3 mL/min, and a detection wavelength of 260 nm. Results Similarity analysis identified 19 characteristic peaks in the fingerprints of Mume Flos. Conclusion The fingerprints established using UPLC are accurate, stable, and reproducible, and provide a scientific basis for further isolation of chemical constituents from Mume Flos and basic research on its effective components.
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