霍山石斛药材HPLC指纹图谱研究
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安徽高校科研创新平台团队项目(皖教秘科〔2015〕49号);安徽省教育厅项目(皖教秘科〔2014〕44号);安徽省科技重大专项计划项目(16030801104)


A Study on the High-performance Liquid Chromatography Fingerprint of Dendrobium huoshanense
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    摘要:

    目的 建立霍山石斛(Dendrobium huoshanense)药材的HPLC指纹图谱,为进一步提高霍山石斛药材的质量提供依据。方法 霍山石斛药材甲醇提取物的分析采用Agilent C18色谱柱(4.6 mm×250 mm,5 μm);流动相甲醇-0.1%磷酸溶液,梯度洗脱;流量为1.0 mL/min;检测波长240 nm;柱温30 ℃。再通过聚类分析、主成分分析和PCA载荷图进行评价。结果 建立了霍山县不同产地的霍山石斛药材指纹图谱,标定了14个共有峰,指认了2个峰,13批霍山石斛药材的指纹图谱的相似度均达到0.850以上;聚类分析、主成分分析和PCA载荷图分析模式均表现出不同产地的霍山石斛药材质量具有差异性。结论 运用3种统计分析方法均能够较好地对指纹图谱进行识别,为霍山石斛药材的采收、质量控制等提供科学依据。

    Abstract:

    Objective To establish the high-performance liquid chromatography (HPLC) fingerprint of Dendrobium huoshanense, and to provide a basis for further improving the quality standard of Dendrobium huoshanense. Methods The methanol extract of Dendrobium huoshanense was analyzed on an Agilent C18 column (4.6 mm×250 mm,5 μm),with a methanol-0.1% phosphoric acid solution as the mobile phase for gradient elution, at a flow rate of 1.0 mL/min, a detection wavelength of 240 nm, and a column temperature of 30 ℃. Cluster analysis, principal component analysis, and PCA load diagram were used to evaluate the results. Results The HPLC fingerprints of Dendrobium huoshanense from different producing places in Huoshan County were established. Fourteen common peaks were obtained, and two of them were identified. The HPLC fingerprint similarity of the 13 batches of Dendrobium huoshanense was greater than 0.850. The cluster analysis, principal component analysis, and PCA load diagram all showed a difference in quality between the Dendrobium huoshanense from different producing places. Conclusion The fingerprints of Dendrobium huoshanense can be identified accurately by the three employed methods of statistical analysis, which provides a scientific basis for the harvesting and quality control of Dendrobium huoshanense.

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刘 刚,俞年军,韩荣春,谢冬梅,刘路路,彭代银,何祥林,张淇军.霍山石斛药材HPLC指纹图谱研究[J].安徽中医药大学学报,2018,37(5):67-70

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  • 在线发布日期: 2018-10-08