左归补髓生血方对再生障碍性贫血小鼠骨髓有核细胞凋亡的影响
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国家中医药管理局中医临床研究基地业务建设科研专项(JDZX2015122)


Effect of Zuogui Busui Shengxue Prescription on the Apoptosis of Bone Marrow Nucleated Cells in Mice with Aplastic Anemia
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    摘要:

    目的 观察左归补髓生血方治疗小鼠再生障碍性贫血的疗效及其对骨髓中有核细胞凋亡的影响。方法 将40只SPF级ICR小鼠随机分正常组、模型组、西药组、中药组。采用二甲磺酸丁酯和环磷酰胺交替给药12 d的方法复制再生障碍性小鼠模型。观察外周血细胞计数以及骨髓组织形态学变化,采用TUNEL法检测各组小鼠骨髓中有核细胞凋亡。结果 与正常组比较,模型组小鼠外周血白细胞、红细胞、血小板计数和血红蛋白浓度明显减低(P<0.05),骨髓有核细胞凋亡率明显升高(P<0.05);光镜下可见小鼠骨髓增生极度减少,红系、粒系、巨核细胞系均明显减少。与模型组比较,中药组以及西药组外周血红细胞、白细胞、血小板计数和血红蛋白浓度明显升高(P<0.05),骨髓有核细胞凋亡率明显降低(P<0.05);光镜下可见骨髓中造血组织比例增多。结论 左归补髓生血方可以有效升高再生障碍性贫血小鼠外周血细胞计数,抑制骨髓中有核细胞的凋亡。

    Abstract:

    Objective To investigate the clinical effect of Zuogui Busui Shengxue Prescription in the treatment of mice with aplastic anemia and its effect on the apoptosis of bone marrow nucleated cells.Methods A total of 40 specific pathogenfree ICR mice were randomly divided into normal group, model group, Western medicine group, traditional Chinese medicine (TCM) group. Busulfan and cyclophosphamide were given alternately for 12 days to establish a mouse model of aplastic anemia. Peripheral blood cell counting and histomorphological changes of bone marrow were observed, and the TUNEL method was used to measure the apoptosis of bone marrow nucleated cells.Results Compared with the normal group, the model group had significant reductions in peripheral blood leukocyte count, red blood cell count, platelet count, and hemoglobin level (P<0.05)and a significant increase in the apoptosis rate of bone marrow nucleated cells (P<0.05),as well as significant reductions in bone marrow hyperplasia,erythroid cells, granulocytes, and megakaryocytic series under a light microscope. Compared with the model group, the TCM group and the Western medicine group had significant increases in peripheral blood leukocyte count, red blood cell count, platelet count, and hemoglobin level (P<0.05) and a significant reduction in the apoptosis rate of bone marrow nucleated cells (P<0.05),as well as a significant increase in the proportion of hematopoietic tissue in bone marrow under the light microscope.Conclusion Zuogui Busui Shengxue Prescription can effectively increase peripheral blood cell counts and inhibit the apoptosis of bone marrow nucleated cells in mice with aplastic anemia.

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张 鹏,何春玲,郭雨晨,李 玮,董昌虎.左归补髓生血方对再生障碍性贫血小鼠骨髓有核细胞凋亡的影响[J].安徽中医药大学学报,2018,37(4):79-82

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  • 在线发布日期: 2018-08-09