反向高效液相色谱法测定覆盆子中3种三萜酸的含量
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安徽省高校自然科学研究重点项目(KJ2015A128)


Determination of Three Triterpene Acids in Rubi Fructus by Reverse-phase High-performance Liquid Chromatography
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    摘要:

    目的 采用反相高效液相色谱法建立覆盆子中三萜类成分(山楂酸、科罗索酸和齐墩果酸)的测定方法,为中药覆盆子的质量评价提供依据。方法 色谱柱:WondaSil C18-WR(250 mm×4.6 mm,5 μm),流动相为乙腈(A)-0.2%磷酸水溶液(B)(9∶1),等度洗脱,柱温为25 ℃,流速0.6 mL/min,检测波长205 nm,进样量10 μL。结果 山楂酸、科罗索酸、齐墩果酸分别在0.156~3.900 μg、0.102~2.55 μg、2.04~51 μg范围内与峰面积呈现良好的线性关系。平均加样回收率分别为95.0%、91.7%、93.8%,RSD分别为2.59%、3.25%、2.86%。结论 该方法简单、可行,可作为覆盆子药材中单一三萜类成分含量的测定方法。

    Abstract:

    Objective To establish a reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of three triterpenes (maslinic acid, corosolic acid, and oleanolic acid) in Rubi Fructus, and to provide a basis for the quality assessment of Rubi Fructus. Methods RP-HPLC was performed on a WondaSil C18-WR column (250 mm × 4.6 mm, 5 μm) with a mobile phase of acetonitrile (A)-0.2% phosphoric acid solution (B) (9∶1) for isocratic elution at a column temperature of 25 ℃, a flow rate of 0.6 mL/min, a detection wavelength of 205 nm, and a sample size of 10 μL. Results The concentrations of maslinic acid, corosolic acid, and oleanolic acid showed a good linear relationship with the peak area within the ranges of 0.156-3.900 μg, 0.102-2.55 μg, and 2.04-51 μg, respectively, with average recovery rates of 95.00%, 91.67%, and 93.80%, respectively (relative standard deviations=2.59%, 3.25%, and 2.86%, respectively). Conclusion This method is simple and feasible and can be used for the determination of triterpenes in Rubi Fructus.

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曹 富,邱晓霞,张 玲,王元媛.反向高效液相色谱法测定覆盆子中3种三萜酸的含量[J].安徽中医药大学学报,2017,36(4):79-82

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  • 在线发布日期: 2017-08-02