Abstract:Objective To establish a reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of three triterpenes (maslinic acid, corosolic acid, and oleanolic acid) in Rubi Fructus, and to provide a basis for the quality assessment of Rubi Fructus. Methods RP-HPLC was performed on a WondaSil C18-WR column (250 mm × 4.6 mm, 5 μm) with a mobile phase of acetonitrile (A)-0.2% phosphoric acid solution (B) (9∶1) for isocratic elution at a column temperature of 25 ℃, a flow rate of 0.6 mL/min, a detection wavelength of 205 nm, and a sample size of 10 μL. Results The concentrations of maslinic acid, corosolic acid, and oleanolic acid showed a good linear relationship with the peak area within the ranges of 0.156-3.900 μg, 0.102-2.55 μg, and 2.04-51 μg, respectively, with average recovery rates of 95.00%, 91.67%, and 93.80%, respectively (relative standard deviations=2.59%, 3.25%, and 2.86%, respectively). Conclusion This method is simple and feasible and can be used for the determination of triterpenes in Rubi Fructus.