Abstract:Objective To investigate the in vitro effect of Salvia miltiorrhiza injection on the plasma protein binding rate of gliquidone. Methods Equilibrium dialysis and high- performance liquid chromatography were used to measure the plasma protein binding rate of gliquidone with the presence or absence of Salvia miltiorrhiza injection. Results There were no significant differences in plasma protein binding rate between high, medium, and low concentrations of gliquidone (12.6, 6.3, and 1.26 μg/mL, respectively) (P>0.05). With the presence of the high- , medium- , and low- concentration Salvia miltiorrhiza injections (750, 375, and 75 mg/mL, respectively), the plasma protein binding rates of gliquidone were(82.6±3.11), (87.3±9.80)%, and (92.6±4.21)%, respectively. With the presence of high- concentration Salvia miltiorrhiza injection, there was a significant reduction in the plasma protein binding rate of gliquidone (P<0.05). Conclusion Salvia miltiorrhiza injection at the dose used in clinical practice does not affect the plasma protein binding rate of gliquidone.