养肝益水颗粒对自发性高血压大鼠肾小球足细胞TGF- β1、p27蛋白及其基因表达的影响
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安徽高校省级自然科学研究项目(kj2013z166)


Effect of Yanggan Yishui Granule on Protein and Gene Expression of Transforming Growth Factor- β1 and p27 in Glomerular Podocytes in Spontaneously Hypertensive Rats
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    摘要:

    目的 从足细胞途径探讨养肝益水颗粒改善高血压肾损害的作用机制。方法 将64只自发性高血压大鼠随机分4组,每组16只,即养肝益水颗粒高、低剂量治疗组,贝那普利组,模型组,并选取16只WKY大鼠作为正常组。检测实验前后各组大鼠的血清肌酐(serum creatinine, SCr)、血尿素氮(blood urea nitrogen, BUN)水平;采用RT- qPCR检测大鼠肾皮质转化生长因子(transforming growth factor beta 1,TGF- β1)、p27 mRNA表达水平;Western blot检测大鼠肾皮质TGF- β1、p27蛋白表达变化。结果 治疗前后各组自发性高血压大鼠SCr、BUN水平显著高于正常组(P<0.05);治疗后,养肝益水颗粒高、低剂量组,西药组大鼠SCr、BUN水平均显著降低(P<0.05),但3组之间比较,差异无统计学意义(P>0.05)。模型组肾皮质p27蛋白、p27 mRNA及TGF- β1 mRNA、TGF- β1蛋白表达水平均明显高于正常组(P<0.05);治疗后,养肝益水颗粒高、低剂量组相比,高剂量组肾皮质p27蛋白及p27 mRNA表达水平均降低(P<0.05);肾皮质TGF- β1 mRNA、TGF- β1蛋白相对表达水平显著降低(P<0.05)。结论 养肝益水颗粒通过下调TGF- β1、p27的表达,调节足细胞的增殖和凋亡,维持足细胞正常形态和结构,从而达到保护肾脏的目的。

    Abstract:

    Objective To investigate the mechanism of action of Yanggan Yishui Granule in improving hypertensive kidney injury via podocytes. Methods A total of 64 spontaneously hypertensive rats were randomly divided into high- and low- dose Yanggan Yishui Granule groups, benazepril group, and model group, with 16 rats in each group; another 16 WKY rats were selected as normal group. The levels of serum creatinine (SCr) and blood urea nitrogen (BUN) were measured before and after the experiment, and RT- qPCR and Western blot were used to measure the mRNA and protein expression of transforming growth factor- β1 (TGF- β1) and p27 in the renal cortex in rats. Results The spontaneously hypertensive rats had significantly higher levels of SCr and BUN than the normal rats before and after treatment (P<0.05). After treatment, the high- and low- dose Yanggan Yishui Granule groups and the benazepril group had significant reductions in the levels of SCr and BUN (P<0.05), but there were no significant differences between the three groups (P>0.05). The model group had significantly higher mRNA and protein expression of p27 and TGF- β1 in the renal cortex than the normal group (P<0.05). After treatment, compared with the low- dose Yanggan Yishui Granule group, the high- dose Yanggan Yishui Granule group had significant reductions in the mRNA and protein expression of p27 and TGF- β1 (P<0.05). Conclusion Yanggan Yishui Granule can protect the kidney through down- regulating the expression of p27 and TGF- β1, regulating the proliferation and apoptosis of podocytes, and maintaining the normal morphology and structure of podocytes.

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杨 帆,戴小华,朱 涛,付 军,王银燕,戚先伟,梁国庆,董 梅,邵正斌.养肝益水颗粒对自发性高血压大鼠肾小球足细胞TGF- β1、p27蛋白及其基因表达的影响[J].安徽中医药大学学报,2017,36(2):56-59

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  • 在线发布日期: 2017-04-07