黄蒲通窍胶囊对血管性痴呆大鼠行为学表现及海马CA1区病理变化的影响
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安徽高校省级自然科学研究重点项目(KJ2012A185);康缘创新基金项目(KYCX201013)


Effects of Huangpu Tongqiao Capsule on Behavioral Manifestation and Pathological Changes in Hippocampal CA1 Region among Rats with Vascular Dementia
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    摘要:

    目的 观察黄蒲通窍胶囊对血管性痴呆(vascular dementia, VaD)大鼠学习记忆功能及海马CA1区病理变化的影响。方法 将Wistar大鼠分为正常组、假手术组、模型组、多奈哌齐组及黄蒲通窍胶囊组。采用双侧颈总动脉永久性结扎法复制VaD大鼠模型。连续灌胃45 d后,采用Morris水迷宫实验观察大鼠的行为学表现,光镜下观察大鼠海马CA1区的病理变化。结果 Morris水迷宫的定位航行实验和空间搜索实验表明,黄蒲通窍组和多奈哌齐组大鼠的逃避潜伏期较模型组显著缩短(P<0.01),跨越平台次数、目标象限次数和目标象限距离均较模型组显著升高(P<0.01)。光镜下观察结果显示,黄蒲通窍组和多奈哌齐组大鼠海马CA1区神经细胞凋亡、坏死较模型组显著减轻。结论 黄蒲通窍胶囊具有改善VaD大鼠学习记忆功能及减轻海马CA1区神经细胞损伤的作用。

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    Objective To investigate the effects of Huangpu Tongqiao Capsule on the learning and memory ability and pathological changes in the hippocampal CA1 region among rats with vascular dementia (VaD). Methods Wistar rats were divided into normal group, sham-operation group, model group, donepezil group, and Huangpu Tongqiao Capsule group. A rat model of VaD was induced by permanent occlusion of bilateral common carotid arteries. After 45 d of intragastric administration, Morris water maze test (MWMT) was performed to observe the behavioral manifestation of rats, and the pathological changes in the hippocampal CA1 region were evaluated under a light microscope. Results The place navigation experiment and space search experiment of MWMT showed that the Huangpu Tongqiao group and donepezil group had significantly shortened escape latency (P<0.01) and significantly increased number of platform crossings, number of target quadrant crossings, and target quadrant ratio (P<0.01), as compared with the model group. The light microscopy showed that the Huangpu Tongqiao group and donepezil group had significantly reduced cell apoptosis and necrosis in the hippocampal CA1 region compared with the model group. Conclusion For rats with VaD, Huangpu Tongqiao Capsule can improve the learning and memory ability and reduce the hippocampal cell damage in the CA1 region.

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王 艳,谢道俊,鲍远程 ,张 娟,储丹晨,韦 彪,孙愘叡,丁伟军,鲍婷婷,王秋烨,赵志勇.黄蒲通窍胶囊对血管性痴呆大鼠行为学表现及海马CA1区病理变化的影响[J].安徽中医药大学学报,2014,33(4):69-72

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  • 在线发布日期: 2014-07-30