芪玉三龙方通过Wnt5a/β-catenin信号通路逆转上皮—间充质转化抑制肺癌细胞侵袭和转移
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国家自然科学基金项目(81874431);安徽省高校优秀拔尖人才培育资助项目(gxbjZD2021057);安徽高校自然科学研究重点项目(KJ2019A0468,KJ2021A0542)


Qiyu Sanlong Prescription Inhibits the Invasion and Metastasis of Lung Cancer Cells by Reversing Epithelial-Mesenchymal Transition via the Wnt5a/β-catenin Signaling Pathway
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    目的 从细胞水平探讨芪玉三龙方是否通过Wnt5a/β-catenin信号通路抑制上皮—间充质细胞转化,抑制肺癌转移。方法 将重组慢病毒转染小鼠肺癌细胞建立稳定过表达Wnt5a的细胞系,同时构建Wnt5a干扰和空载细胞株。qRT-PCR法检测Wnt5a、β-catenin mRNA的表达水平。CCK8、EdU实验检测肺癌细胞的增殖活性,Transwell实验检测肺癌细胞的迁移和侵袭能力。Western blot法检测Wnt5a、β-catenin、c-Myc、Cyclin D1、Vimentin、N-cadherin、E-cadherin、Snail蛋白表达水平。结果 与对照组比较,芪玉三龙方组Wnt5a、β-catenin、c-Myc、Cyclin D1蛋白表达水平下降(P<0.05),细胞增殖活性、侵袭和迁移能力显著降低(P<0.05)。Wnt5a过表达组中Vimentin、N-cadherin、Snail表达水平增加(P<0.05),E-cadherin蛋白表达水平下降(P<0.05),细胞增殖活性、侵袭和迁移能力显著增强(P<0.05);Wnt5a干扰组Vimentin、N-cadherin、Snail表达水平下降(P<0.05),E-cadherin蛋白表达水平升高(P<0.05),细胞增殖活性、侵袭和迁移能力显著下降(P<0.05),明显抑制EMT的发生。芪玉三龙方处理后,Wnt5a过表达组Wnt5a、β-catenin、c-Myc、Cyclin D1、Vimentin、N-cadherin、Snail表达水平下降(P<0.05),E-cadherin蛋白表达水平升高(P<0.05),细胞增殖活性、侵袭和迁移能力得到抑制。结论 芪玉三龙方可能通过Wnt5a/β-catenin信号通路逆转上皮—间充质转化,抑制肺癌细胞增殖和转移。

    Abstract:

    Objective To investigate whether Qiyu Sanlong Prescription can inhibit the metastasis of lung cancer by inhibiting epithelial-mesenchymal transition via the Wnt5a/β-catenin signaling pathway at the cellular level.Methods Mouse lung cancer cells were transfected with recombinant lentivirus to establish a cell line with the stable expression of Wnt5a,and Wnt5a interference and empty vector cell lines were established.qRT-PCR was used to measure the expression of Wnt5a and β-catenin.CCK8 and EdU assay were used to measure the proliferative activity of cells,and Transwell assay was used to measure the migration and invasion abilities of cells.Western blot was used to measure the protein expression levels of Wnt5a,β-catenin,c-Myc,Cyclin D1,Vimentin,N-cadherin,E-cadherin,and Snail.Results Compared with the control group,the Qiyu Sanlong Prescription group had significant reductions in the protein expression levels of Wnt5a,β-catenin,c-Myc,and Cyclin D1(P<0.05) and significantly lower proliferative activity and invasion and migration abilities of cells(P<0.05).The Wnt5a overexpression group had significant increases in the expression levels of Vimentin,N-cadherin,and Snail(P<0.05),a significant reduction in the protein expression level of E-cadherin(P<0.05),and significant enhancement of the proliferative activity and invasion and migration abilities of cells(P<0.05); the Wnt5a interference group had significant reductions in the expression levels of Vimentin, N-cadherin,and Snail(P<0.05);a significant increase in the protein expression level of E-cadherin(P<0.05),and significant reductions in the proliferative activity and invasion and migration abilities of cells(P<0.05).After treatment with Qiyu Sanlong prescription,the Wnt5a overexpression group had significant reductions in the expression levels of Wnt5a,β-catenin,c-Myc,Cyclin D1,Vimentin,N-cadherin,and Snail(P<0.05),a significant increase in the expression level of E-cadherin(P<0.05),and significant inhibition of the proliferative activity and invasion and migration abilities of cells(P<0.05).Conclusion Qiyu Sanlong prescription inhibits the proliferation of tumor cells by reversing epithelial-mesenchymal transition via the Wnt5a/β-catenin signaling pathway.

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张 裕,童佳兵,李泽庚,高雅婷.芪玉三龙方通过Wnt5a/β-catenin信号通路逆转上皮—间充质转化抑制肺癌细胞侵袭和转移[J].安徽中医药大学学报,2024,43(2):81-88

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  • 在线发布日期: 2024-04-07