电针心经穴位对急性心肌缺血模型大鼠内侧隔核白细胞介素-2、JunB蛋白及c-fos表达水平的影响
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国家自然科学基金项目(81674058)


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    目的 观察电针心经对急性心肌缺血(acute myocardial ischemia,AMI)大鼠内侧隔核白细胞介素(interleukin,IL)-2水平、JunB蛋白及c-fos表达水平的影响,探究内侧隔核在针刺抗AMI中的作用及机制。方法 将大鼠分为伪手术组、模型组和电针组,每组6只;结扎冠状动脉左前降支复制AMI大鼠模型;电针组大鼠选取手少阴心经“神门—通里”段进行干预,每次30 min,每日1次,连续电针3 d,刺激电流为1 mA,频率为2 Hz;伪手术组、模型组大鼠不进行电针干预。采用ELISA法检测大鼠大脑内侧隔核IL-2水平,Western blot法检测大鼠大脑内侧隔核区JunB蛋白表达水平,免疫荧光法检测大鼠大脑内侧隔核区c-fos免疫反应阳性神经元表达水平。结果 与伪手术组比较,模型组大鼠大脑内侧隔核区IL-2、JunB蛋白水平显著升高(P<0.05),c-fos免疫反应阳性神经元数和平均吸光度(optical density,OD)值显著增加(P<0.05);与模型组比较,电针组大鼠大脑内侧隔核区IL-2、JunB蛋白水平显著降低(P<0.05),c-fos免疫反应阳性神经元数和平均OD值显著减少(P<0.05)。结论 内侧隔核参与电针心经抗AMI的作用,其机制可能与电针降低大脑内侧隔核区IL-2、JunB蛋白及c-fos表达水平有关。

    Abstract:

    Objective To investigate the effect of acupuncture at the heart meridian on the expression levels of IL-2,JunB protein,and c-fos in the medial septal nucleus of rats with acute myocardial ischemia (AMI),as well as the role and mechanism of the medial septal nucleus in acupuncture against AMI.Methods The rats were divided into sham-operation group,model group,and electroacupuncture group, with 6 rats in each group.Ligation of the left anterior descending coronary artery was performed to establish a rat model of AMI.The rats in the electroacupuncture group were given intervention at the "Shenmen-Tongli" segment of the heart meridian of hand-Shaoyin for 30 minutes each time,once a day for 3 consecutive days,with an intensity of 1 mA and a frequency of 2 Hz,while those in the sham-operation group and the model group were not treated with electroacupuncture.ELISA was used to measure the level of IL-2 in the medial septal nucleus of rats;Western blot was used to measure the protein expression level of JunB in the medial septal nucleus of rats;immune of luorescence assay was used to measure the expression of c-fos-positive neurons in the medial septal nucleus of rats.Results Compared with the sham-operation group,the model group had significant increases in the levels of IL-2 and JunB protein (P<0.05) and the number and average optical density (OD) of c-fos-positive neurons (P<0.05) in the medial septal nucleus of rats.Compared with the model group,the electroacupuncture group had significant reductions in the levels of IL-2 and JunB protein (P<0.05) and the number and average OD of c-fos-positive neurons (P<0.05) in the medial septal nucleus of rats. Conclusion The medial septal nucleus participates in the effect of electroacupuncture at the heart meridian in the treatment of AMI,possibly by reducing the expression levels of IL-2,JunB protein,and c-fos-positive neurons in the medial septum nucleus.

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李锦航,周美启.电针心经穴位对急性心肌缺血模型大鼠内侧隔核白细胞介素-2、JunB蛋白及c-fos表达水平的影响[J].安徽中医药大学学报,2024,43(2):38-41

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  • 在线发布日期: 2024-04-07