冠心平含药血清对氧化低密度脂蛋白诱导的RAW264.7巨噬细胞泡沫化的影响
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江苏省重点研发计划项目(BE2020683);江苏省中医药科技发展计划重点项目(ZD201906);江苏省中医院创新发展基金项目(Y2019CX20)


Effect of Serum Containing Guanxinping on the Foaming of RAW264.7 Macrophages Induced by Oxidized Low-density Lipoprotein
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    目的 观察冠心平含药血清对氧化低密度脂蛋白( oxidized low-density lipoprotein, ox-LDL)诱导的RAW264.7巨噬细胞泡沫化的调控作用。方法 将RAW264.7巨噬细胞分为对照组,模型组,冠心平含药血清低、中、高剂量组,以80 μg/mL ox-LDL刺激24 h,诱导巨噬细胞泡沫化模型,采用不同浓度冠心平含药血清进行干预。油红O染色观察巨噬细胞内脂滴形成;ELISA法检测巨噬细胞内游离胆固醇(free cholesterol,FC)与胆固醇酯(cholesterol esterase,CE)的表达水平;实时荧光定量PCR法检测巨噬细胞清道夫受体A(scavenger receptor-A,SR-A)和CD36 mRNA表达水平。结果 冠心平中、高剂量能够显著减少巨噬细胞泡沫化;低剂量显著增加巨噬细胞FC水平(P<0.05),高剂量显著降低巨噬细胞CE水平(P<0.05);冠心平高剂量显著增加SR-A mRNA表达水平(P<0.05),低、中、高剂量均显著增加CD36 mRNA表达水平(P<0.05)。结论 冠心平能够抑制巨噬细胞的泡沫化,这或许是冠心平治疗动脉粥样硬化的潜在机制之一。

    Abstract:

    Objective To investigate the regulatory effect of serum containing Guanxinping on the foaming of RAW264.7 macrophages induced by oxidized low-density lipoprotein (ox-LDL). Methods RAW264.7 macrophages were divided into control group, model group, and low-, middle-, and high-dose serum containing Guanxinping groups. RAW264.7 macrophages were stimulated by 80 μg/mL ox-LDL for 24 hours to establish a model of macrophage foaming, and different concentrations of serum containing Guanxinping were used for intervention. Oil red O staining was used to observe the formation of lipid droplets in macrophages; ELISA was used to measure the expression of free cholesterol (FC) and cholesterol esterase (CE) in macrophages; quantitative real-time PCR was used to measure the mRNA expression of SR-A and CD36 in macrophages. Results Middle- and high-dose Guanxinping significantly reduced the foaming of macrophages. Low-dose Guanxinping significantly increased the level of FC in macrophages (P<0.05), and high-dose Guanxinping significantly reduced the level of CE in macrophages (P<0.05). High-dose Guanxinping significantly increased the mRNA expression level of SR-A (P<0.05), and low-, middle-, and high-dose Guanxinping significantly increased the mRNA expression level of CD36 (P<0.05). Conclusion Guanxinping can inhibit the foaming of macrophages, which may be one of the potential mechanisms of Guanxinping in the treatment of atherosclerosis.

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周冠进,严 飞,孙玉婷,陈韦凯,掌琳惠,袁冬平,刘福明.冠心平含药血清对氧化低密度脂蛋白诱导的RAW264.7巨噬细胞泡沫化的影响[J].安徽中医药大学学报,2021,40(4):79-82

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  • 在线发布日期: 2021-08-14