排脓散汤剂和散剂中有效成分生物利用度的比较研究
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国家自然科学基金项目(81874348);新安医学教育部重点实验室开放课题(2020xayx11);安徽中医药大学探索性基金资助项目(2017HXTS42)


Bioavailability of Effective Constituents in Decoction Versus Powder of Painong San
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    摘要:

    目的 比较排脓散的汤剂与散剂中有效成分的药物代谢动力学参数,为排脓散的剂型选择提供依据。方法 以栀子苷为内标,采用UPLC- MS/MS法测定大鼠血液中芍药苷、橙皮苷、新橙皮苷和柚皮苷的含量,用乙酸乙酯法提取后,制备样品,然后使用Themo Scientific Syncronis C18色谱柱(2.1 mm×100 mm,2.7 μm)在35 ℃下进行色谱分离,以水和乙腈为流动相,流速0.3 mL/min,进行负离子模式的多反应监测,用于测定血浆中标准品和内标。结果 4种有效成分含量在0.2~500 ng/mL范围内与峰面积呈良好的线性关系,提取回收率、稳定性、精密度均良好且达到测定要求。大鼠经单次给药后,与汤剂比较,排脓散散剂可显著增加橙皮苷的生物利用度(P<0.05),而不能显著增加新橙皮苷、柚皮苷和芍药苷的生物利用度(P>0.05)。结论 排脓散散剂的生物利用度可能更优于汤剂。

    Abstract:

    Objective To investigate the differences in pharmacokinetic parameters of effective constituents between the decoction and power of Painong San, and to provide a basis for selecting the dosage form of Painong San. Methods With geniposide as the internal standard, ultra- performance liquid chromatography- tandem mass spectrometry was used to determine the content of paeoniflorin, hesperidin, neohesperidin, and naringin in rat blood. Samples were prepared after extraction using ethyl acetate, and then chromatographic separation was performed on a Thermo Scientific Syncronis C18 column (2.1 mm×100 mm, 2.7 μm) with a mobile phase of water and acetonitrile, at a temperature of 35 ℃ and a flow rate of 0.3 mL/min. Multiple reaction monitoring with the negative ion mode was performed to measure the standard substances and the internal standard in plasma. Results The four effective constituents showed a good linear relationship with the peak area within the range of 0.2- 500 ng/mL, with good extraction recovery, stability, and precision, which met the determination requirements. Compared with the decoction of Painong San after a single administration in rats, the powder significantly increased the bioavailability of hesperidin (P<0.05), with no significant increases in the bioavailability of neohesperidin, naringin, and paeoniflorin (P>0.05). Conclusion The powder of Painong San may have better bioavailability than the decoction.

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李嘉诚,张孟孟,芮雪琳,杨 晔,徐 莉,江倩倩,尹登科.排脓散汤剂和散剂中有效成分生物利用度的比较研究[J].安徽中医药大学学报,2021,40(3):80-85

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  • 在线发布日期: 2021-06-16