目的 探讨木丹颗粒对高糖环境下人血管内皮细胞核转录因子E2相关因子2（neclear factor erythroid 2-related factor 2，Nrf2）/抗氧化反应元件（antioxidant response element，ARE）信号通路及血管内皮生长因子（vascular endothelial growth factor，VEGF）的影响。方法 将健康雄性Wistar大鼠30只随机分为正常组、模型组、木丹颗粒组，每组10只，制备含药血清；将正常组含药血清加入正常糖浓度培养内皮细胞，模型组及木丹颗粒组分别加入高糖培养内皮细胞；采用RT-PCR、Western blot分别检测Nrf2、血红素氧合酶-1(heme oxygenase-1, HO-1)的mRNA及蛋白表达水平；采用ELISA法检测各组细胞上清液中VEGF含量。结果 与正常组比较，模型组Nrf2、HO-1的mRNA及蛋白表达均显著上调（P<0.05），VEGF的含量显著增加（P<0.05）；与模型组比较，给药组Nrf2、HO-1的mRNA及蛋白表达水平均显著上调（P<0.05），VEGF的含量显著减少（P<0.05）。结论 〖JP2〗木丹颗粒对高糖环境下人血管内皮细胞的保护作用机制可能与上调Nrf2/ARE信号通路的表达、降低VEGF含量有关。
Objective To investigate the effect of Mudan Granule on the nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant responsive element (ARE) signaling pathway and vascular endothelial growth factor (VEGF) in human vascular endothelial cells under high-glucose environment. Methods A total of 30 healthy male Wistar rats were randomly divided into normal group, model group, and Mudan Granule group, with 10 rats in each group, and the drug-containing serum was prepared. In the normal group, the drug-containing serum was added to the endothelial cells cultured at a normal glucose concentration, and in the model group and the Mudan Granule group, the drug-containing serum was added to the endothelial cells cultured at a high glucose concentration. RT-PCR and Western blot were used to measure the mRNA and protein expression of Nrf2 and heme oxygenase-1 (HO-1), and ELISA was used to measure the content of VEGF in the supernatant of each group. Results Compared with the normal group, the model group had significant increases in the mRNA and protein expression of Nrf2 and HO-1 (P<0.05) and the content of VEGF (P<0.05). Compared with the model group, the Mudan Granule group had significant increases in the mRNA and protein expression of Nrf2 and HO-1 (P<0.05) and a significant reduction in the content of VEGF (P<0.05). Conclusion Mudan Granule exerts a protective effect on human vascular endothelial cells under high-glucose environment, possibly by upregulating the expression of the Nrf2/ARE signaling pathway and reducing the content of VEGF.
李可月,李 都,王 镁.木丹颗粒对高糖环境下人血管内皮细胞Nrf2/ARE信号通路的影响[J].安徽中医药大学学报,2020,39(1):55-59复制