超高效液相色谱法比较鲜川芎和川芎药材中6种成分含量
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国家自然科学基金项目(81573720)


Comparison of Six Active Components Between Fresh and Dried Rhizoma Ligustici Chuanxiong Based on Ultra-high-performance Liquid Chromatography
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    摘要:

    目的 采用超高效液相色谱法(ultra-high performance liquid chromatography, UPLC)比较鲜川芎和川芎药材中6种活性成分(川芎嗪、阿魏酸、洋川芎内酯I、洋川芎内酯H、洋川芎内酯A、藁本内酯)的含量差异,为该药材的产地加工提供实验依据。方法 采用75%甲醇超声提取,UPLC测定鲜川芎和川芎药材中6种活性成分,根据样品处理条件计算质量分数并进行比较。流动相为乙腈(A)-0.1%甲酸水溶液(B),梯度洗脱,流速为0.2 mL/min,检测波长为294 nm,柱温30 ℃,进样体积2 μL。结果 经检测,阿魏酸、藁本内酯、洋川芎内酯A为鲜川芎和川芎药材的共有成分,鲜川芎中未检测到洋川芎内酯I及洋川芎内酯H。鲜川芎样品中各成分的质量分数为阿魏酸0.003 9%、洋川芎内酯A 0.006 4%、藁本内酯0.54%;川芎药材中各成分的质量分数为阿魏酸0.088%、洋川芎内酯I 0.20%、洋川芎内酯H 0.031%、洋川芎内酯A 0.76%、藁本内酯 1.3%。结论 川芎药材与鲜川芎中化学成分存在差异,推测在川芎的产地加工及贮藏过程中存在成分的转化。

    Abstract:

    Objective To investigate the differences in the content of six active components (ligustrazine, ferulic acid, senkyunolide I, senkyunolide H, senkyunolide A, and ligustilide) between fresh and dried Rhizoma Ligustici Chuanxiong based on ultra-high-performance liquid chromatography (UPLC), and to provide an experimental basis for the processing of this medicinal material in producing areas. Methods Ultrasonic extraction was performed with 75% methanol, and UPLC was used to measure the content of the above 6 active components in fresh and dried Rhizoma Ligustici Chuanxiong. Mass fraction was calculated based on sample treatment conditions, and a comparative analysis was performed. UPLC was performed with a mobile phase of acetonitrile (A)-0.1% formic acid solution (B) for gradient elution, at a flow rate of 0.2 mL/min, a detection wavelength of 294 nm, a column temperature of 30 ℃, and an injection volume of 2 μL. Results Ferulic acid, ligustilide, and senkyunolide A were detected in both fresh and dried Rhizoma Ligustici Chuanxiong, and senkyunolide I and senkyunolide H were not detected in fresh Rhizoma Ligustici Chuanxiong. In fresh Rhizoma Ligustici Chuanxiong, the mass fractions of ferulic acid, senkyunolide A, and ligustilide were 0.003 9%, 0.006 4%, and 0.54%, respectively; in dried Rhizoma Ligustici Chuanxiong, the mass fractions of ferulic acid, senkyunolide I, senkyunolide H, senkyunolide A, and ligustilide were 0.088%, 0.20%, 0.031%, 0.76%, and 1.3%, respectively. Conclusion There are differences in chemical components between dried and fresh Rhizoma Ligustici Chuanxiong, and it is speculated that component conversion occurs during processing in producing areas and storage of Rhizoma Ligustici Chuanxiong.

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陈 冉,尹丹丹,杨 沫,王运来,尹登科,许 钒.超高效液相色谱法比较鲜川芎和川芎药材中6种成分含量[J].安徽中医药大学学报,2019,38(6):84-88

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  • 在线发布日期: 2019-11-25