目的 建立一测多评方法比较无硫和低硫白芷药材中5种香豆素类成分含量的差异，并验证方法准确性和可行性。方法 以欧前胡素为内参物，建立相对校正因子。计算4种香豆素成分含量，同时与外标法计算结果进行比较，验证该方法的可行性。结果 相对校正因子在不同实验条件下RSD均在3.0%以内，且一测多评法测定结果与外标法测定结果无显著差异。结论 一测多评法可以用于无硫和低硫白芷药材中5种香豆素类成分含量测定。
Objective To investigate the difference in the content of five coumarins between sulfur-free and sulfur-smoked Radix Angelicae Dahuricae based on the quantitative analysis of multi-components by single marker (QAMS), and to evaluate the accuracy and feasibility of this method. Methods Imperatorin was used as the internal reference substance and its relative correction factors with the other four components were established. The content of the other four coumarins was calculated and compared with the results calculated by the external standard method to verify the feasibility of the method. Results The RSD of the relative correction factor was within 3.0 % in different experimental conditions and there was no significant difference between the results of QAMS and the external standard method. Conclusion QAMS can be used for simultaneous determination of the content of five coumarins in sulfur-free and sulfur-smoked Radix Angelicae Dahuricae.
张 强,宋 宝,谢冬梅,王国凯.一测多评法测定无硫和低硫白芷中5种香豆素类成分的含量[J].安徽中医药大学学报,2019,38(6):79-83复制