目的 观察灵芪颗粒对环磷酰胺致免疫功能低下小鼠和正常小鼠免疫功能的影响。方法 小鼠腹腔注射环磷酰胺，复制免疫功能低下模型，观察灵芪颗粒对小鼠血白细胞数、淋巴细胞数、T淋巴细胞转化率、NK细胞活性、碳粒廓清和免疫器官脏器系数的影响；观察灵芪颗粒对正常小鼠溶血素、溶血空斑数和2,4-二硝基氟苯介导的皮肤迟发性超敏反应的影响。结果 灵芪颗粒能明显提高环磷酰胺致免疫功能低下小鼠的脾T淋巴细胞转化能力、NK细胞活性和碳粒廓清能力，增加外周血白细胞数和淋巴细胞数，并减轻免疫器官（脾、胸腺）的萎缩。灵芪颗粒能明显增加正常小鼠的半数溶血值和溶血空斑数，并增强2,4-二硝基氟苯介导的小鼠迟发性超敏反应。结论 灵芪颗粒能增强小鼠的特异性免疫和非特异性免疫。
Objective To investigate the immunoregulatory effect of Lingqi Granule in mice with immunocompromise induced by cyclophosphamide and normal mice. Methods The mice were given intraperitoneal injection of cyclophosphamide to establish a mouse model of immunocompromise, and the effects of Lingqi Granule on leukocyte count, lymphocyte count, T lymphocyte transformation rate, natural killer (NK) cell viability, carbon particle clearance, and coefficient of immune organ were observed. The effects of Lingqi Granule on hemolysin, number of hemolytic plaques, and delayed-type hypersensitivity in skin mediated by 2,4-dinitrofluorobenzene in normal mice were observed. Results In the mice with immunocompromise induced by cyclophosphamide, Lingqi Granule significantly improved spleen T lymphocyte transformation ability, NK cell viability, and carbon particle clearance ability, increased peripheral blood leukocyte and lymphocyte counts, and reduced atrophy of immune organs (including the spleen and thymus). In the normal mice, Lingqi Granule significantly increased the half hemolytic concentration and number of hemolytic plaques and enhanced delayed-type hypersensitivity mediated by 2,4-dinitrofluorobenzene. Conclusion Lingqi Granule can enhance specific and non-specific immunity in mice.