经皮穴位电刺激不同时间对雷公藤甲素致大鼠急性肝损伤的影响
DOI:
作者:
作者单位:

作者简介:

通讯作者:

基金项目:

江苏省中医药管理局课题(LB13049)


Protective Effects of Transcutaneous Electrical Acupoint Stimulation for Different Times on Acute Hepatic Injury Induced by Triptolide in Rats
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
    摘要:

    目的 观察经皮穴位电刺激(transcutanclus electrical acupoint stimulation,TEAS)不同刺激时间对雷公藤甲素致大鼠急性肝损伤的保护作用。方法 将50只SD大鼠随机分为空白组、模型组、TEAS Ⅰ组(刺激20 min),TEAS Ⅱ组(刺激30 min)、TEAS Ⅲ组(刺激40 min),以雷公藤甲素灌胃法复制急性肝损伤模型。TEAS各组刺激“足三里”穴,每日1次,连续5 d。观察肝脏组织形态学变化,检测血清天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)及肝组织丙二醛(malondialdehyde,MDA)、肿瘤坏死因子- α(tumor necrosis factor- α,TNF- α)、白介素- 10(interleukin- 10,IL- 10)、硫化氢的含量。结果 模型组大鼠肝索排列紊乱,肝窦扩张,肝细胞水肿、脂肪变性、坏死;各TEAS组肝脏病理变化较模型组减轻。与空白组比较,模型组大鼠血清ALT、AST及肝组织MDA、TNF- α含量明显升高(P<0.05),硫化氢含量明显降低(P<0.05),肝组织IL- 10含量无明显变化(P>0.05)。与模型组比较,TEAS Ⅰ、Ⅱ、Ⅲ组大鼠血清AST、ALT含量和肝组织MDA、TNF- α含量明显降低(P<0.05)或呈降低趋势(P>0.05),硫化氢含量明显升高(P<0.05)。其中TEAS Ⅲ组与TEAS Ⅰ、Ⅱ组各指标比较,差异均具有统计学意义(P<0.05)。结论 TEAS能减轻雷公藤甲素对肝组织的损伤,且较长时间(40 min)刺激的保护作用更为明显。

    Abstract:

    Objective To observe the effect of transcutaneous electrical acupoint stimulation (TEAS) for different times on acute hepatic injury caused by triptolide in rats. Methods A total of 50 Sprague- Dawley rats were equally and randomly divided into normal group, model group, TEAS Ⅰ group (20- min stimulation), TEAS Ⅱ group (30- min stimulation), and TEAS Ⅲ group (40- min stimulation). The rat model of acute hepatic injury was established by treatment with triptolide by gavage. Since the first day of modeling, the TEAS groups were stimulated at Tsusanli (ST36) once daily for 5 days. Hepatic histomorphological changes were observed and levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum and malondialdehyde (MDA), tumor necrosis factor- α (TNF- α), interleukin- 10 (IL- 10), and hydrogen sulfide in liver tissues were measured. Results In the model group, disordered hepatic cords, sinusoidal expansion, hepatocyte edema, hepatic steatosis, and necrosis were observed. These pathological changes were alleviated in the TEAS groups. In comparison with those in the normal group, levels of ALT, AST, MDA, and TNF- α were significantly higher (P<0.05) and the level of hydrogen sulfide was significantly lower (P<0.05) in the model group. There was no significant difference in IL- 10 level between the two groups (P>0.05). Compared with those in the model group, the levels of AST, ALT, MDA, and TNF- α in TEAS groups were significantly lower (P<0.05) or there was a decreasing trend in these indices (P>0.05); the level of hydrogen sulfide was significantly higher in the TEAS groups (P<0.05). Significant differences in these indices were observed between the TEAS Ⅲ group and the TEAS Ⅰ or Ⅱ group (P<0.05). Conclusion TEAS can reduce the injury to rat liver caused by triptolide, and the protective effect of long- time TEAS (40 min) is more remarkable compared with short- time stimulation (20 min or 30 min).

    参考文献
    相似文献
    引证文献
引用本文

言 枫,程秫泺,李广林,陈 昊,王建美,顾一煌,曾庆琪.经皮穴位电刺激不同时间对雷公藤甲素致大鼠急性肝损伤的影响[J].安徽中医药大学学报,2015,34(4):54-57

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2015-08-06