安徽高校省级自然科学研究项目（KJ 2009 B022）；安徽中医药大学自然科学研究项目（2013zr013）
目的 观察延胡索不同生长期新生块茎和母块茎的特点。方法 用定点连续观察和石蜡切片法，跟踪观察延胡索块茎形成过程及新生块茎、母块茎的生长变化。结果 由地下茎产生的新生块茎生长起点在母株的茎节处，其形成需要某些必要的条件；母块茎退化成更新块茎的周皮，其脱落需要几个月的时间；栽培品产生双生及多生块茎较野生品常见；块茎中含有荧光生物碱。结论 新生块茎的形成方式具多样性；退化的周皮对更新块茎具保护作用；栽培品无性繁殖能力较强；荧光生物碱在延胡索成熟块茎中的分布广泛，没有聚集性。
Objective To observe the features of new tubers and mother tubers in different growth stages of Corydalis yanhusuo. Methods We traced the tuber formation process of Corydalis yanhusuo, as well as the growth of new tubers and mother tubers, by real-time observation and paraffin sectioning. Results The new tubers produced by the underground stem grew from the stem nodes of mother plant, and their formation needed some necessary conditions. The new tubers were also reproduced from the mother tubers; the mother tubers degenerated into the periderm of the reproduced tubers and finally fell off a couple of months later. It was more common for cultivars to produce twin tubers and multiple tubers compared with wild varieties. Tubers contained fluorescent alkaloids. Conclusion New tubers are formed in a variety of ways. The periderm from degenerated mother tubers can protect reproduced tubers. Cultivars have a strong ability of asexual reproduction. Fluorescence alkaloids are distributed homogeneously in the mature tubers of Corydalis yanhusuo, and no aggregation is observed.